유방암종에서의 HER-2/neu 유전자 검색을 위한 발색제자리부합화에 관한 연구
Chromogenic In Situ Hybridization Analysis to Determinate HER-2/neu Status in Breast Carcinoma
김지연, 최경운, 정윤주, 곽희숙, 배영태,
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김지연 ( Kim Jee-Yeun )
부산대학교 의과대학 병리학교실
최경운 ( Choi Kyung-Un )
부산대학교 의과대학 병리학교실
정윤주 ( Jung Youn-Joo )
부산대학교 의과대학 외과학교실
곽희숙 ( Kwak Hee-Suk )
부산대학교 의과대학 외과학교실
배영태 ( Bae Young-Tae )
부산대학교 의과대학 외과학교실
KMID : 0371320040660060447
Abstract
Purpose: The determination of HER-2/neu gene amplification lias become necessary for the selection of breast cancer patients to undergo anti-HER-2/neu therapy, using a humanized monoclonal antibody. Chromogenic in situ hybridization (CISH) detection of the HER-2/neu gene, a newly developed method, utilizes a robust and unique-sequence DNA probe labeled with digoxygenin, which is sequentially incubated with antidigoxygenin fluorescein, antifluorescein peroxidase and diaminobenzidine. The aim of this study was to establish a CISH assay for the detection of HER-2/neu amplification. The results were compared with those of the immunonistochemistry (IHC) methods, most frequently used for detecting HER-2/neu alteration.
Methods: CISH was performed in 4 groups of infiltrating breast carcinomas. Each group was comprised of 20 cases in which the HER-2/neu stati had previously been scored on a four value scale: 0, 1+, 2+ and 3+ by IHC. The results of CISH and IHC were compared for each tumor group. The HER-2/reu gene amplification detected by CISH was thpically visualized as large DAB-stained clusters or by many dots in the nucleus.
Results: The concordance between the CISH and IHC was 95% (κ=0.901). Three IHC-positive cases (score 2+) showed no gene amplification and one IHC-negative case (score 1+) Showed gene amplification by CISH.
Conclution: The current study showed excellent agreement between the CISH and IHC methods. CISH is an accurate, practical and economical approach for determining the HER-2/neu stati in breast carcinomas. It is also a useful methodology for confirming the IHC results in paraffin-embedded tumor samples, so offers a promising alternative to IHC in a routine diagnostic setting.
키워드
유방암종;HER-2/neu;발색제자리부합화;면역조직화학염색;Breast Carcinoma;Chromogenic In Situ Hybridization;CISH;Immunohistochemistry
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