죽상동맥경화에 의한 말초혈관 폐쇄성 질환에 있어서 Angiotensin-Converting Enzyme 유전자 Insertion/Deletion과 E-selectin 유전자 S128R의 다형성
Angiotensin Converting Enzyme Gene Insertion/Deletion Polymorphism and E-selectin S128R Polymorphism in Peripheral Atherosclerotic Occlusive Disease
정금오, 권정남, 박동은, 김정중, 문형배, 소병준,
소속 상세정보
정금오 ( Jung Gum-O )
원광대학교 의과대학 외과학교실
권정남 ( Kwon Jong-Nam )
원광대학교 의과대학 외과학교실
박동은 ( Park Dong-Eun )
원광대학교 의과대학 외과학교실
김정중 ( Kim Jeong-Joong )
원광대학교 의과대학 해부학교실
문형배 ( Moon Hyung-Bae )
원광대학교 의과대학 병리학교실
소병준 ( So Byung-Joon )
원광대학교 의과대학 외과학교실
KMID : 0371320040660060508
Abstract
Purpose: Studies concerning the relationship between gene polymorphisms and potentially implicated cardiovascular disease have produced conflicting findings, in part due to differences in ethnic background between populations. These led us to evaluate the impact of polymorphisms in the ACE and E-selectin genes on peripheral artery atherosclerosis in a Korean population.
Methods: We studied 92 male patients (median age: 65.9, range: 48~82) with severe peripheral atherosclerosis documented by angiography and ABI (ankle brachial index). The control group comprised 290 healthy persons (male 216, female 64, median age 61.3, range 20~90) without symptoms for peripheral vascular disease. The blood samples were stored at -20℃ until DNA was ready to be extracted. The inorganic procedure for DNA extraction was based on the method described by Miller et al. The ACE and E-selectin polymorphisms were detected by polymerase chain reaction (PCR) amplification.
Results: The distribution of ACE genotypes of the patient group was as follows: Ⅱ, 34 (37.0%); ID, 46 (50.0%); and DD, 12 (13.0%). It was not significantly different from that of the control subjects: Ⅱ, 104 (37.1 %); ID, 133 (47.6%); and DD, 43 (15.3%) (P=0.80). The allele frequencies of the patient group were as follows: I, 114 (62.0%); and D, 70 (38.0%). It was not significantly different from that of the control subjects: I, 341 (60.9%); and D, 219 (39.1%) (P=0.80). The frequencies of E-selectin genotypes in the patient group were as follows: Ser/Ser 85 (93.4%); Ser/Arg, 6 (6.6); and Arg/Arg, 0 (0%). It was not significantly different from that of the control subjects: Ser/Ser, 262 (93.6%); Ser/Arg, 18 (6.4%); and Arg/Arg, 0 (0%) (P=0.95). In addition, the allele frequencies of the patient group were as follows: Ser, 176 (96.7%); and Arg, 6 (3.3%). It was not significantly different from that of the control subjects: Ser, 542 (96.8%); and Arg 18 (3.2%) (P=0.95).
Conclusion: The I/D polymorphism of the ACE gene and E-selectin S 128R polymorphism were not significantly different between the atherosclerotic patient group and the normal control group in Koreans.
키워드
Peripheral artery atherosclerosis;Genetic polymorphism;Angiotensin-converting enzyme gene;ACE gene;E-selectin gene;말초동맥죽상경화증;유전자 다형성;안지오텐신 전환효소 유전자;E-셀렉틴 유전자
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