한국인의 대동맥류와 말초동맥 폐색성 질환에서 클라미디아 폐렴균의 검출양상에 대한 연구
Detection of Chlamydia pneumoniae in Korean Patients with Abdominal Aortic Aneurysm or Peripheral Arterial Occlusive Disease
민승기, 황유진, 강혜진, 하승연, 이승준, 정인묵, 하종원, 정중기, 김상준,
소속 상세정보
민승기 ( Min Seung-Kee )
가천의과학대학교 외과학교실
황유진 ( Hwang You-Jin )
가천의과학대학교 생명과학부
강혜진 ( Kang Hye-Jin )
가천의과학대학교 생명과학부
하승연 ( Ha Seung-Yeon )
가천의과학대학교 해부병리학교실
이승준 ( Lee Seung-Jun )
강원대학교 의과대학 내과학교실
정인묵 ( Jung In-Mok )
서울대학교 의과대학 외과학교실
하종원 ( Ha Jong-Won )
서울대학교 의과대학 외과학교실
정중기 ( Chung Jung-Kee )
서울대학교 의과대학 외과학교실
김상준 ( Kim Sang-Joon )
서울대학교 의과대학 외과학교실
KMID : 0371320040670040296
Abstract
PURPOSE: Chlamydia pneumoniae (CP) infection seems to be related to atherosclerotic diseases. A prospective sero- epidemiologic study was performed to analyze the relationship between CP infection and peripheral vascular disease in Korean patients. The aims of this study were to find the prevalences of CP antibody in the serum and CP antigens in the vascular tissues, and to analyze the differences between several disease groups.
METHODS: Our subjects included a total of 61 patients (76 vascular tissues) who had undergone operative procedures for peripheral vascular diseases. They were classified into 3 groups: Group 1; 14 abdominal aortic aneurysm, Group 2; 15 atherosclerosis obliterans, and Group 3; 32 varicose vein cases. The CP antibody titers were determined using the microimmunofluorescence test (MIF) and the CP antigen in the vascular tissues with a semi-nested polymerase chain reaction (PCR) and an in situ hybridization technique (ISH).
RESULTS: The prevalences of chronic or past CP infection from the MIF (IgG antibody titer > or = 1:32) in Groups 1, 2 and 3 were 78.6, 73.3, and 68.8% respectively, but with statistically significant differences. The prevalences of PCR- positive tissues in Groups 1, 2 and 3 were 21.4, 6.7, and 0% respectively. There was no CP DNA detected in the venous tissue. CP DNA was detected more frequently in aneurysmal disease than atherosclerosis obliterans, but this was not statistically significant(p=0.265). In comparison with the varicose veins, aortic aneurysms showed a significantly higher PCR positivity ratio (p=0.002), and a similar result was seen with ISH. There was no relationship between CP antigen positivity and the known risk factors for atherosclerosis.
CONCLUSION: A high prevalence of CP antibodies was observed in the serum of Korean patients with vascular disease, which matched that in western populations. CP DNA was also detected in atherosclerotic tissues, which was especially high in aneurysmal disease, implying a possible causative role of CP infection in the pathogenesis of the atherosclerotic disease. This is the first report on the prevalence of CP in vascular tissues in Korean population.
키워드
클라미디아 폐렴균;말초혈관질환;대동맥류;죽상경화성 폐색증;정맥류;Chlamydia pneumoniae;Peripheral vascular disease;Aortic aneurysm;Atherosclerosis obliterans;Varicose vein
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