Comparison of the Inhibitory Effects of Retinoic Acid Derivatives on Development of Squamous Metaplasia from Rat Mammary Epithelial Organoids Cultured in Matrigel
백기주, 전성실, 윤정현, 김남득,
소속 상세정보
백기주 ( Paik Kee-Joo )
부산대학교 약학대학
전성실 ( Jeon Seong-Sill )
부산대학교 약학대학
윤정현 ( Yoon Jeong-Hyun )
부산대학교 약학대학
김남득 ( Kim Nam-Deuk )
부산대학교 약학대학
KMID : 0603519970020020155
Abstract
To compare the inhibitory effects of retinoic acids derivatives among all-tracts, 13-cis and 9-cis retinoic acids on the development of squamous metaplasia (SQM) from rat mammary epithelial cells (RMEC), female F344 rat mammary organoids were cultured in reconstituted basement membrane, Matrigel, under either a complete hormone medium (CHM) or serum-free mammary epithelium growth medium (MEGM). From the culture, five different types of multicellular colonies were developed: stellate, ductal, webbed, squamous, and lobulo-ductal colonies. Organoids cultured in CHM without retinoids gave rise to fewer such SQM (∼5%) than those in MEGM without retinoids (∼16%). Formation of SQM was completely suppressed when three different retinoic acids derivatives [10-9 M], all-trans, 13-cis and 9-cis RAs, were added to CHM. However, a few SQM were still observed in cultures in MEGM with the added retinoids [10-6 M]. In the presence of retinoids in culture medium, lobulo-ductal colonies, one of well-differentiated structures, were increased with the augmentation of retinoids concentrations. However, there were no difference in the differentiation inducing activities among three different retinoic acid derivatives.
키워드
Squamous metaplasia; all-trans retinoic acid; 13-cis retinoic acid; 9-cis retinoic acid
원문 및 링크아웃 정보
등재저널 정보