Bile-Acid Induced Apoptosis in HL-60 Leukemia Cells
김광록, 강창모, 백진현, 이유미, 김규원,
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김광록 ( Kim Kwang-Rok )
부산대학교 분자생물학과
강창모 ( Kang Chang-Mo )
부산대학교 생물학과
백진현 ( Baek Jin-Hyen )
부산대학교 분자생물학과
이유미 ( Lee You-Mie )
부산대학교 분자생물학과
김규원 ( Kim Kyu-Won )
부산대학교 분자생물학과
KMID : 0603519980030020107
Abstract
We investigated that lithocholic acid (LCA) or ursodeoxycholilc acid (UDCA), components of bile salt, induced apoptosis in HL-60 cells. The growth of HL-60 cells was reduced by these compounds in a time- and a concentration-dependent manners. Moreover, the degree of DNA fragmentation was increased depending on the treatment time and molar concentration of LCA or UDCA on agarose gel electrophoresis. Morpllologically, we examined the formation of membrane-enclosed apoptotic bodies in LCA or UDCA-treated HL-60 cells by haematoxylin/eosin staining. The expression of c-myc was down-regulated after 24 h treatment of LCA or UDCA In case of bcl-2, there was no change of the expression after 24 h treatment of LCA or UDCA. The DNA fragmentation was reduced when HL-60 cells were treated with 0.5 μM cycloheximide (CHX) and LCA or UDCA. Therefore, bile acids, LCA and UDCA, induced apoptosis in HL-60 cells, which required dr novo protein synthesis.
키워드
HL-60 leukemia cells; Apoptosis; LCA; UDCA; c-myc
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