MMP-9/SEAP 벡터를 이용한 새로운 암전이 억제제 검색법의 개발
A New Assay System for Analysing the Inhibitors of Tumor Invasion and Metastasis Using MMP-9/SEAP Vector
차희재, 정주원, 이유미, Seiki Motoharu, 김옥희, 김규원,
소속 상세정보
차희재 ( Cha Hee-Jae )
부산대학교 분자생물학과
정주원 ( Jung Joo-Won )
부산대학교 분자생물학과
이유미 ( Lee You-Mie )
부산대학교 분자생물학과
( Seiki Motoharu )
일본 동경대학교 의과학연구소 암세포연구부
김옥희 ( Kim Ok-Hee )
국립독성연구소
김규원 ( Kim Kyu-Won )
부산대학교 분자생물학과
KMID : 0603519980030020115
Abstract
During the steps of tumor metastasis, matrix metalloproteinase-9 (MMP-9) is one of the most important enzymes and acts a key role to degrade the extracellular matrix. Therefore suppressing the expression of MMP-9 may inhibit the tumor invasion and metastasis. In this study, we developed the assay system to analyze the expression of MMP-9 by constructing the MMP-9 reporter vector and confirming the action of reporter protein by SEAP assay. Furthermore, we confirmed the expression pattern of MMP-9 is similar to that of Northern blot and gelatin zymogram assay. This assay system enables to detect the expression of MMP-9 by treating only simple methods. Therefore, we can apply this system to screen the MMP-9 inhibitors in various candidate compounds and factors.
키워드
MMP-9; SEAP vector; Invasion; Metastasis
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