두툽상어에서 Membrane Type-3 Matrix Metalloproteinase (MT3-MMP) 유전자의 클로닝
Cloning of Membrane Type-3 Matrix Metalloproteinase (MT3-MMP) Gene from Scyliorhinus Torazame
김명순, 배문경, 김지태, 송현석, 안미영, 김종원, 임운기, 김규원, 김영진,
소속 상세정보
김명순 ( Kim Myung-Soon )
부산대학교 분자생물학과
배문경 ( Bae Moon-Kyoung )
부산대학교 분자생물학과
김지태 ( Kim Ji-Tae )
부산대학교 분자생물학과
송현석 ( Song Hyun-Seok )
부산대학교 분자생물학과
안미영 ( Ahn Mee-Young )
부산대학교 분자생물학과
김종원 ( Kim Jong-Won )
부산대학교 분자생물학과
임운기 ( Lim Woon-Ki )
부산대학교 분자생물학과
김규원 ( Kim Kyu-Won )
부산대학교 분자생물학과
김영진 ( Kim Yung-Jin )
부산대학교 분자생물학과
KMID : 0603519990040010017
Abstract
Matrix metalloproteinases (MMPs) are zinc-binding endopeptides that collectively degrade the constituent macromolecules of the extracellular matrix. Thus, the MMPs are thought to play important roles during organ morphogenesis, embryonic development and pathological processes. However, MMP genes, including MT-MMPs, were previously cloned and their functions were studied in mammalians such as human, mouse, and chick, but not yet in fishes. In this study, we cloned the 5’region and 3’region of shark MT3-MMP gene and analyzed their sequences in comparison with those of other species. The nucleotide sequence of the 450 bp PCR product by 5’RACE is 74% identical to MT3-MMP genes of human, rat, and chick, by the Genbank nucleotide data base search. Also, 5’region of shark MT3-MMP contains 37 bp 5’UTR (untranslated region), start codon (ATG), the putative signal peptide, and cystein switch domain (RXKR motif). The nucleotide sequence of the 1,400 bp 3’LD-PCR product shows 80% homologies to the MT3-MMP of them. 5’region of shark MT3-MMP contains stop codon (TGA) and 76 bp of the putative transmembrane domain.
키워드
Membrane type matrix metalloproteinase; Shark
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