7,12-dimethylbenz[a]anthracene (DMBA)에 의해 유발된 랫드의 유선종양에서 DNA 배수성 양상
DNA Ploidy Patterns in Mammary Gland Tumors Induced by 7,12-dimethylbenz[a]anthracene (DMBA) in Rats
강진석, 안병우, 김대중, 김기석, 남기택, 한범석, 배종희, 박응복, 장동덕,
소속 상세정보
강진석 ( Kang Jin-Seok )
식품의약품안전청 국립독성연구원 독성병리과
안병우 ( Ahn Byeong-Woo )
식품의약품안전청 국립독성연구원 독성병리과
김대중 ( Kim Dae-Joong )
식품의약품안전청 국립독성연구원 독성병리과
김기석 ( Kim Ki-Sok )
식품의약품안전청 국립독성연구원 독성병리과
남기택 ( Nam Ki-Taek )
식품의약품안전청 국립독성연구원 독성병리과
한범석 ( Han Beom-Seok )
식품의약품안전청 국립독성연구원 독성병리과
배종희 ( Bae Jong-Hee )
제주대학교 수의과대학
박응복 ( Bak Ung-Bok )
서울대학교 수의과대학
장동덕 ( Jang Dong-Deuk )
식품의약품안전청 국립독성연구원 독성병리과
KMID : 0603519990040010025
Abstract
DNA cytometry has frequently been used to characterize DNA ploidy pattern in relation to clinical and histopathological findings. Anueploidy of tumor cells has been considered to be related with poor prognosis of patients. This study was carried out to examine the relationship between DNA ploidy patterns obtained by flow cytometry (FCM) & static cytometry (SCM) and histopathological observations of mammary gland tumors induced by 7,12-dimethylbenz[a]anthracene (DMBA) in rats. Eighteen from the total of 40 SD rats treated with DMBA (10 mg/kg) developed 39 tumor masses. Tumor incidence was 45.0% and tumor multiplicity was 0.97. Adenocarcinoma was most predominant subtype among DMBA-induced tumors. In 35 cases of malignant tumors, the incidence of aneuploid tumor was slightly higher in SCM than in FCM. In comparison of histopathological findings with DNA ploidy patterns aneuploidy was correlated with the grade of mitotic rate in malignant tumors (P<0.05). Morphological parameters such as nuclear areas and perimeter of nuclei were increased tendency with aneuploidy. Our results suggest that increased tendency of malignancy might be correlated with the degree of aneuploidy in mammary gland tumors of rats.
키워드
Rat; DMBA; Mammary tumor; Histopathological observation; DNA ploidy
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