Sarcoma 180 이식마우스에서 Interleukin-2, 4, 10의 변화
Changes of Interleukin-2, 4, 10 in Sarcoma 180-transplanted Mice
이원기, 김광혁, 정영기, 박건영,
소속 상세정보
이원기 ( Lee Won-Ki )
고신대학교 의과대학 미생물학교실
김광혁 ( Kim Kwang-Hyuk )
고신대학교 의과대학 미생물학교실
정영기 ( Jeong Yong-Kee )
동의대학교 미생물학과
박건영 ( Park Kun-Young )
부산대학교 식품영양학과
KMID : 0603519990040010034
Abstract
Experiments were undertaken to investigate the effect of cancer cell, sarcoma 180 (S180) on production of interleukin(IL)-2, 4, and 10 in BALB/C mice. Splenocytes of normal mice were incubated with S 180 cells. The culture supernatant were collected at 2,4, 6,24, and 72 hr and assessed for IL-2,4, and IL-10 production by enzyme-linked immunosorbent assay. The production of IL-2 in the culture supernatant of adherent cells exposed with S 180 was not detected, but it increased at 24 and 72 hr in culture with non-adherent or whole splenocyte. The production of IL-4 in the culture supernatant of S 180, adherent, non-adherent, or whole splenocyte was detected, but by co-culture with S 180 it showed significant increase at 72 hr. Also, the production of IL-10 in the culture supernatant of S 180, adherent, non-adherent, or whole splenocytes was detected. By exposure with S 180 it increased to 10-80 times in comparison with the control. Normal mice were transplanted with S 180 into peritoneum. Sera and peritoneal fluids(PF) were collected at 2, 4, 6, and 24 hr. The production of IL-2 in sera was not detected, but in PF it was a little. The production of IL-4 in sera or PF was detected, but it showed the significant decrease in comparison with the control. The production of IL-10 in sera was detected in transplantation or control group, and it increased in transplantation group. The production of IL-10 in PF showed the increase at 24 hr. By exposure of S 180 tumor cells in immuno-competent cells or transplantation with S 180 into mouse, IL-4 decreased in sera and PF, but IL-10 showed the remarkable increase in the culture supernatant of splenocytes and in the sera. These data suggest that the unbalanced conditions such as suppression of IL-4 production and overproduction of IL-10 if vivo may be responsible for dysfunction of immune system.
키워드
Mouse; Sarcoma 180; Interleukin-2; Interleukin-4; Interleukin-10
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