In vitro 및 in vivo에서 된장의 암에방 효과 -2.된장 추출물이 Sarcoma-180 이식 마우스의 혈청 및 간내 주요 효소활성에 미치는 효과-
Cancer Preventive Effects of Doenjang in vitro and in vivo -2. Effects of Doenjang Extracts on the Changes of Serum and Liver Enzyme Activities in Sarcoma-180 Transplated Mice-
손미현, 문숙희, 최종원, 박건영,
소속 상세정보
손미현 ( Son Mi-Hyun )
부산대학교 식품영양학과
문숙희 ( Moon Suk-Hee )
경남정보대학 식품과학계열
최종원 ( Choi Jong-Won )
경성대학교 약학과
박건영 ( Park Kun-Young )
부산대학교 식품영양학과
KMID : 0603519990040030143
Abstract
The human diet contains a great variety of carcinogens as well as many natural anticarcinogens (cancer-preventive substances). Many antioxiant nutrients in food have been identified as anticarcinogens. Numerous epidemiological data have shown that intake of green yellow vegetables which were rich in vitamin C, -βcarotetene and vitamin E-reduced risk of cancer. The interest of green juice made of green yellow vegetables is rapidly increasing in Korea. However, the study on it has not been extensively studied. the objectives of this study were to investigate biological activities such as antioxidant effect, cancer cell growth inhibitory effect of methanol fraction obtained from green juices (Angelica keiskei, kale, carrot) and their antioxidant vitamin contents. Antioxidant effect was determined by the peroxide value (POV), which measures the oxidation of linoleic acid during storage. The PO of control increased up to 156 meq/㎏ of the 7 days, but the POV of linoleic acid in Angelica keiskei, kale and carrot’s methanolic extracts was 9.1, 4.7 and 37.8 meq/㎏, respectively. From these results, methanolic extracts from green juice showed significantly stronger antioxidant activity than the control (p<0.05). Especially, kale juice’s methanolic extracts had stronger antioxidant activity than the others. The inhibitory effect of methanolic extracts on the growth of cancer cells, neuro 2a, was significantly higher than the control. The growth rates of cancer cells in the medium containing green juice extracts was significantly inhibited as the extract concentration increased. When the extract of kale juice were added to more than 0.25 ㎎/mL or extracts of other juices were added to more than 1 ㎎/mL, the growth of cancer cells was inhibited of other juices were added to more than 1 ㎎/mL, the growth of cancer cells was inhibited by 100%. Consequently, the extract of kale juice was shown to provide the greatest functional properties among the vegetables tested. The extract of kale juice contained vitamin E and vitamin E and vitamin C significantly higher than the other extracts.
키워드
Doenjang extracts; Serum and liver enzymes; Sarcoma-180 cells; Mouse
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