애엽 추출물 밑 이에 함유된 Eupatilin의 아폽토시스를 통한 HL-60 세포 사멸 유도및 종양 촉진 억제 작용
Introduction of Apoptosis in HL-60 Cells and suppression of Mouse Skin Tumor Promotion by Artemisia Asiatica (Composite) and Its Ingredient Eupatilin
서효정, 손미원, 김원배,
소속 상세정보
서효정 ( Seo Hyo-Joung )
서울대학교 약학대학
손미원 ( Son Mi-Won )
동아제약주식회사 연구소
김원배 ( Kim Won-Bae )
동아제약주식회사 연구소
KMID : 0603519990040030167
Abstract
Extracts of the whole herb of Artemisia asiatica Nakai (Composite) have been used in traditional Oriental medicine for the treatment of inflammation, diarrhea, and many circulatory disorders. Since tumor promotion is closely associated with inflammatory processes, A. asiatica (mugwort) is anticipated to exert a potential anti-tumor promoting activity. In the present work, we assessed the protective effects of the methanol extract of A. asiatica on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin tumor promotion as well as inflammation in female ICR mice. Pretreatment of the shaven dorsal skin of mice with A. asiatice half an hour before topical application of TPA inhibited ear edema at 5 h. A. asiatica was also found to significantly suppress the TPA-induced ornithine decarboxylase activity and interleukin-1 transcription in mouse epidemics. Likewise, TPA-induced expression of epidermal cyclxooygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) was mitigated by tropical application of the methanol extract of A. asiatica. In another study, A. asiatica and its pharmacologically active principle 5,7-dihydroxy-3’,4’,6-trimethoxyflavon (eupatilin) exhibited inhibitory effects on viability and DNA synthesis in HL-60 cells. The anti-proliferative activity of A. asiatica and eupatilin was attributable to their apoptosis inducing capability as determined by characteristic morphological changes, nuclear condensation, DNA fragmentation and in situ terminal end-labeling of fragmented DNA (TUNELL). In conclusion, A. asiatica attenuates the mouse skin tumor promotion and induces apoptosis in cultured cancer cells. Taken together, above findings suggest that this medicinal plant possessed the substantial cancer chemopreventive and therapeutic potential.
키워드
Apoptosis; Antitumor promotion; Artemisia asiatica; Eupatilin
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