형질전환동물세포주(Big Blue® Transgenic Mouse Cell Line)를 이용한 돌연변이 및 항돌연변이시험
Antimutagenic and Mutagenic Test in Big Blue® Transgenic Mouse Embryonic Cell
엄미옥, 한의식, 김종원, 손수정, 류병규, 변은경, 최재수, 오혜영,
소속 상세정보
엄미옥 ( Eom Mi-Ok )
식품의약품안전청 국립독성연구소 유전독성과
한의식 ( Han Eui-Sik )
식품의약품안전청 국립독성연구소 유전독성과
김종원 ( Kim Jong-Won )
식품의약품안전청 국립독성연구소 유전독성과
손수정 ( Sohn Soo-Jung )
식품의약품안전청 국립독성연구소 유전독성과
류병규 ( Ryu Byung-Kyu )
식품의약품안전청 국립독성연구소 유전독성과
변은경 ( Byun Eun-Kyung )
식품의약품안전청 국립독성연구소 유전독성과
최재수 ( Choi Jae-Sue )
부경대학교 식품생명과학과
오혜영 ( Oh Hye-Young )
식품의약품안전청 국립독성연구소 유전독성과
KMID : 0603520000050030117
Abstract
Transgenic animal cell with a λshuttle vector containing a lac I target gene is useful as a short-term in vitro mutagenesis assay. The gene is recovered from the treated cell by exposing mouse genomic DNA to in vitro packaging extracts and plating the rescued phage on agar plates containing 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal). Phage with mutations in the lac I gene form blue plaques, whereas phage with a nonmutated lac I form colorless plaques. In order to estabilish this new transgenic Technology, we studied the mutagenicity of the direct-acting alkylating agent and indirect mutagen benzo[a]pyrene (B[a]P) in the Big Blue® transgenic mouse embryonic cell line. MNU or B[a]P caused an induction of mutations over negative control, 1% DMSO and we confirmed the reproducibility of the transgenic mutagenicity assay. Also, dose- dependent response has been observed in cells treated with B[a]P. When the antimutagenic effect of Ecklonia strolonifera, an edible brown algae having a strong antioxidizing activity, on MNU-induced mutation was evaluated, the methanol extract of Ecklonia strolonifera showed reduction of mutant frequency in cells treated with MNU.
키워드
Transgenic mouse embryoonic cell;N-methyl-N-nitrosourea (MNU);Benzo[a]pyrene (B[a]P);Ecklonia stolonifera;Mutation assay;Anti-mutagenicity
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