Galangin의 생체외 및 생체내 부정기 DNA합성 억제 효과
Inhibition Effects of Galangin against 2-AAF Induced in vitro and in vivo Unscheduled DNA Synthesis
김종원, 한의식, 엄미옥, 박미선, 이진주, 정해권, 오혜영,
소속 상세정보
김종원 ( Kim Jong-Won )
식품의약품안전청 국립독성연구소 유전독성과
한의식 ( Han Eui-Sik )
식품의약품안전청 국립독성연구소 유전독성과
엄미옥 ( Eom Mi-Ok )
식품의약품안전청 국립독성연구소 유전독성과
박미선 ( Park Mi-Sun )
식품의약품안전청 국립독성연구소 유전독성과
이진주 ( Lee Jin-Joo )
식품의약품안전청 국립독성연구소 유전독성과
정해권 ( Jung Hai-Kwan )
식품의약품안전청 국립독성연구소 유전독성과
오혜영 ( Oh Hye-Young )
식품의약품안전청 국립독성연구소 유전독성과
KMID : 0603520010060020076
Abstract
Unscheduled DNA synthesis (UDS) test is well validated and known as one of genetic toxicity tests. UDS provides information as an indicative of DNA synthesis and DNA repair. The purpose of this study is that we would like to help for many researchers to evaluate test substances (drug, food additive and so on) correctly and its application to assess antimutagenic effect. For this purpose, according to SOP, we have performed in vitro and in vivo UDS test in male rat hepatocyte treated with 2-acetylaminofluorene (2-AAF) as a positive control compound. Thereafter, we have selected galangin which is one of anti-oxidants and flavonoids, and performed UDS test about galangin. And we have studied antimutagenic effect of galangin against 2-AAF using in vitro and in vivo UDS test in male rat hepatocyte. In the in vitro UDS test, 2-AAF showed significantly increase UDS from 10-8M to 10-5M. According to above results, we selected 10-6M 2-AAF as a co-treatment concentration which was shown highest increase UDS. The percentage of UDS in treated all concentration of galangin was similar to that of negative control. When the galangin co-treated with 2-AAF, galangin inhibited 2-AAF-induced UDS up to 80%. In the in vivo UDS test, 2-AAF showed significantly increase UDS in 0.75, 7.5, 75 ㎎/㎏. According to above results, we selected 75 ㎎/㎏ 2-AAF as a co-treatment concentration which was shown highest increase UDS. The galangin didn’t show any increase compared with negative control in all doses. When the galangin co-treated with 2-AAF, galangin inhibited 2-AAF-induced UDS up to 83%.
키워드
in vitro unscheduled DNA synthesis; in vivo unscheduled DNA synthesis; 2-acetylaminofluorene; Galangin
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