녹차의 화학적 암 예방기전 연구
Effects of Green Tea-Drinking on CYPIAs in Different Tissues
양미희, 이인선, 요시가와 마시히로, 아라시다니 케이이찌, 가와모토 도시히로,
소속 상세정보
양미희 ( Yang Mi-Hi )
서울대학교 의과대학 예방의학교실
이인선 ( Lee In-Seon )
계명대학교 응용과학부
요시가와 마시히로 ( Yoshikawa Masahiro )
일본산업의과대학 산업보건학부 제2환경관리학과
아라시다니 케이이찌 ( Arashidani Keiichi )
일본산업의과대학 산업보건학부 제2환경관리학과
가와모토 도시히로 ( Kawamoto Toshihiro )
일본산업의과대학 의학부 위생학교실
KMID : 0603520020070010001
Abstract
To study chemopreventive mechanism of green tea (GT), we studied the effects of GT-drinking on hepatic, pulmonary, and renaI CYP1As against 3-methylcholand1rene(MC), which is a CYP1A-inducing arcinogen. Pre-and concurrent 2% GT drinking (1ldays) was conducted in arylhyrocarbon receptor (AR) responsive C57 BL/6 mice. As results, GT drinking reduced MC-induced pulmonary ethoxyresorufin-O- demethyIase (EROD)-activity, which is a probe for total CYPlAs-activity including CYPlAl and lA2. In contrast, GT drinking enhanced MC-induced hepatic EROD-activity-EROD activity in kidney was lower than those in lung or liver and GT drinking did not affect MC-induced renal EROD activity. These results suggest that the different effects of GT on CYPlAs are diverse in different tissues. Arylhydmcarbon hydroxyIase (AHH) activity, which is known to ref1ect CYPlAl activity, was not different according to GT drinking in lung, liver, or kidney.
Therefore, CYPlA2 or other microsomal enzymes rather than CYPlA1 might be affected by GT in lung, liver and kidney. However, the changes of EROD activity by GT were not confirmed with CYP1A antibody. Our results suggest that susceptibility to chemopreventive GT against CYPlA-biotfansformed carcinogens might be different in different tissues.
키워드
Green tea; CYPlA; Chemoprevention
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