계배융모막에서 인슐린-유사 성장인자 II (IGF-II)의 혈관신생 활성조절에 관한 연구
Regulation of Angiogenic Activity of Insulin-like Growth Factor II (IGF-II) in Chick Chorioallantoic Membrane
배명호, 배수경, 이옥희, 권유욱, 김문범, 오창근, 장호선, 권경술, 김규원,
소속 상세정보
배명호 ( Bae Myung-Ho )
부산대학교 분자생물학과
배수경 ( Bae Soo-Kyung )
부산대학교 분자생물학과
이옥희 ( Lee Ok-Hee )
부산대학교 분자생물학과
권유욱 ( Kwon Yoo-Wook )
부산대학교 분자생물학과
김문범 ( Kim Moon-Bum )
부산대학교 의과대학 피부과학교실
오창근 ( Oh Chang-Keun )
부산대학교 의과대학 피부과학교실
장호선 ( Jang Ho-Sun )
부산대학교 의과대학 피부과학교실
권경술 ( Kwon Kyung-Sool )
부산대학교 의과대학 피부과학교실
김규원 ( Kim Kyu-Won )
서울대학교 약학대학
KMID : 0603520020070010019
Abstract
Insulin-like growth factor II (IGF-II) is highly expressed during hepatocarcinogenesis and psoriasis. It has also angiogenic activity in quantiative chick chorioallantoic membrane (CAM) assay. However, the regulating factor of angiogenic activity of IGF-II is largely unknown. This study was undertaken to examine the control mechanism of angiogenic activity of IGF-II during the development of hepatocellular carcinoma and psoriasis. In this study, we examined angiogenic activity of IGF-II within HepG2 con-ditioned media by immunoprecipitaion and CAM assay. The EGF potentiated the angiogenic activity of IGF-II synergistically while heparin had no effect on the angiogenic activity of IGF-II. This effect of EGF may due to increased-activity of endothelial cell migration. However, EGF had no effect on the proliferating activity of endothelial cells. Finally, we demonstrated that IGF-IIR induced the expression of IGF-II. These results may examine the development mechanism of the hepatocellular carcinoma and psoriasis.
키워드
Insulin like growth factor-II; Angiogenesis; Endothelial cell; Psoriasis
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