발생초기단계 혈관형성에 관련된 Egr-l 결합단백질의 검색과 그 작용에 관한 연구
Identification and Functional Analysis of Egr-l-interacting Proteins Involved in Vasculogenesis During Early Stage of Development
배명호, 배문경, 정주원, 김세희, 정철호, 배수경, 김규원,
소속 상세정보
배명호 ( Bae Myung-Ho )
부산대학교 분자생물학과
배문경 ( Bae Moon-Kyoung )
서울대학교 약학대학
정주원 ( Jeong Joo-Won )
서울대학교 약학대학
김세희 ( Kim Se-Hee )
서울대학교 약학대학
정철호 ( Jeong Chul-Ho )
서울대학교 약학대학
배수경 ( Bae Soo-Kyung )
부산대학교 분자생물학과
김규원 ( Kim Kyu-Won )
서울대학교 약학대학
KMID : 0603520020070020094
Abstract
Egr-I is a zinc finger transcription factor and one of immediate early response genes. Egr-l is upregulated in response to a wide variety of mitogenic and nonmitogenic stimuli, including peptide growth factors, shear stress, urea, hypotonicity and hypoxia. To identify novel Egr-l interacting protein, we performed yeast two-hybrid screening using Egr-l baits. In this study, we identified that proteasome subunit αtype 3 (PSMA3) and protein inhibitor of activated STAT protein (PIASy) interacts specifically with Egr-l protein by yeast two-hybrid screening. In addition, we found that the stability of Egr-l was increased under hypoxic condition. these results suggested the importance of Ｏ₂tension in the degradation processes of Egr-l.
키워드
Egr-1; Two-hybrid; Proteasome subunit α type 3; ProtehI inhibitor of activated STAT protein y; Hypoxia
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