bFGF가 유도하는 혈관신생과정 에서 HMGI(Y)의 작용에 관한 연구
Involvement of High Mobility Group Protein I(Y) in Basic Fibroblast Growth Factor-induced Angiogenesis
구선영, 김명숙, 백진현, 문은정, 배문경, 김규원,
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구선영 ( Koo Sun-Young )
부산대학교 분자생물학과
김명숙 ( Kim Myoung-Sook )
부산대학교 분자생물학과
백진현 ( Baek Jin-Hyen )
부산대학교 분자생물학과
문은정 ( Moon Eun-Jeung )
부산대학교 분자생물학과
배문경 ( Bae Moon-Kyoung )
부산대학교 분자생물학과
김규원 ( Kim Kyu-Won )
서울대학교 약학대학
KMID : 0603520020070020101
Abstract
Expression of High mobility group protein (HMG)I(Y), architectural transcription factor is elevated in a lot of cancer and highly proliferating cells such as fibroblast and smooth muscle cells. HMGI(Y) has been implicated in the control of cell proliferation and gene expression. Here we observed the expression of HMGI(Y) in proliferating Human Umbilical Vein Endothelial CeIls (HUVEC). When basic Fib개blast Growth Factor (bFGF), a well-known angiogenic factor, induced cell proliferation, HMG I(Y) mRNA was increased by RT-PCR. To investigate role of HMGI(Y) in cell proliferation, we used distamycin A, natural antibiotics which is also known as competitor of HMGI(Y). In MTT assay, bFGF induced proliferation of HUVECs but distamycin A with bFGF decreased cell proliferation. Moreover, distamycin A blocked bFGF-induced angiogenesis in vivo mouse matrigel plug assay and chorioallantoic membrane (CAM) assay. Taken together, we suggest that HMGI(Y) plays an important role in bFGF-induced angiogenesis especially through controlling of cell proliferation.
키워드
High mobility group protein I(Y); basic Fibroblast Growth Factor;Angiogenesis; Proliferation; Distamycin A
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