글루타치온 의존성 Selenoprotein W의 생물학적 활성
Biological Function of Selenoprotein W: a Glutathione-Dependent Antioxidant
김익영,
소속 상세정보
김익영 ( Kim Ick-Young )
고려대학교 생명과학대학 생명과학부
KMID : 0603520030080010030
Abstract
The function of selenoprotein W (Se-W) was investigated by cloning the corresponding cDNA from mouse brain and expressing it in CHO ceIls and Hl299 human lung cancer cells. Overexpression of Se-W markedly reduced the sensitivity of both cell lines to H2O2 cytotoxicity. The intracellular peroxide concentration of the transfected cells was lower than that of the parental cells in the absence or presence of extracellular H2O2. The resistance to oxidative stressconferred by Se-W was dependent on glutathione. Expression of Se-W mutants in which selenocysteine-13 or cysteine-37 was replaced by serine did not confer resistance to H2O2, implicating these residues in the antioxidant activity of Se-W in vivo.
키워드
Selenium; Selenoprotein W; GSH; Antioxidant
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