HT-29 세포의 세포고사를 유도하는 Echinomycin과 그 새로운 유도체의 MAP Kinase 신호전달경로의 역할
The Role of MAP Kinase Pathways in Echinomycin and Vovel Analogue-induced Apoptosis of HT-29 Cells
박수정, Park Ju-Youn, 심광용, Kim Yun-Bong, 김성호, 김용해, 김수기,
소속 상세정보
박수정 ( Park Su-Jung )
연세대학교 원주의과대학 미생물학교실
( Park Ju-Youn )
연세대학교 원주의과대학 미생물학교실
심광용 ( Shim Kwang-Yong )
연세대학교 원주의과대학 내과학교실
( Kim Yun-Bong )
KAIST 화학과
김성호 ( Kim Seong-Ho )
KAIST 화학과
김용해 ( Kim Yong-Hae )
KAIST 화학과
김수기 ( Kim Soo-Kie )
연세대학교 원주의과대학 미생물학교실
KMID : 0603520030080020063
Abstract
Echinomycin, typical DNA minor groove binder had comparable efficacy compared to 5-FU in phase II trial for colon cancer treatment. To improve echinomycin’s drawback, we synthesized YK-2000 series (echinomycin analogues). Echinomycin and YK-2000 enabled to induce the apoptosis on HT-29 colorectal cancer cell line. Apoptosis in HT-29 cell triggered by echinomycin and YK-2000 were proven through DNA laddering, PARP (poly-(ADP-ribose) polymerase) cleavage and flow cytometric analysis. Such an apoptotic pattern displayed by YK-2000 was quite different from that of echinomycin. Next, to explore signaling pathway of echinomycin and YK-2000, we examined phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2), stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAP kinase. However, it is not clearly that the mechanism of cancer cell death induced by echinomycin and YK-2000. Here, we report that one of major apoptotic signaling pathway induced by echinomycin and YK-2000 may be MAP inase pathway in HT-29 human colon cancer cells.
키워드
Echinomacin;YK-2000;Apoptosis;MAP kINASE;PARP
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