호장근 추출물의 Helicobacter pylori에 대한 생육억제 및 인간 위암세포주에 대한 유전적 손상 유도
Growth Inhibition of Helicobacter pylori and Induction of Genetic Damage on Human Gastric Cancer Cell by Reynoutria elliptica(Koidz) Migo. Extracts
박정현, 김현정, 이지원, 임효권, 이인선,
소속 상세정보
박정현 ( Park Jung-Hyun )
계명대학교 전통미생물자원개발 및 산업화연구센터
김현정 ( Kim Hyun-Jeong )
계명대학교 전통미생물자원개발 및 산업화연구센터
이지원 ( Lee Ji-Won )
계명대학교 식품가공학과
임효권 ( Im Hyo-Gwon )
계명대학교 식품가공학과
이인선 ( Lee In-Seon )
계명대학교 전통미생물자원개발 및 산업화연구센터
KMID : 0603520030080020081
Abstract
Helicobacter pylori are related to gastric cancer. After screening of antimicrobial activities from various plants methanol extracts against H. pylori KCTC2948, we found Reynoutria elliptica methanol extract and further fractions have significant antibacterial effects especially hexane fr. and CHCl3 fr. The growth inhibition effects on the gastric cancer cell lines such as KatoIII and AGS cells were investigated with MTT. After 48 hr treatments R. elliptica extracts and fractions were shown growth inhibition upto 70～ 80% at 0.4～1 mg/ml concentrations. The growth inhibition effects was higher AGS than KATO III. We applied comet assay to measure the DNA damage in the individual cells and exposed time course at IC50. All fractions have shown to time dependent on DNA damage and these fractions also observed higher than 80% in DNA strand breaks. These DNA strand breaks were transient, reaching a maximum level after 2 hr of exposure. In conclusion, we found that R. elliptica has in vitro growth-inhibiting and apoptosisinducing effects on gastric cancer cells.
키워드
Helicobacter pylori; Reynoutria elliptica; Comet assay; DNA damage
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