겨우살이 첨가 김치의 발효양상과 항돌연변이 및 암세포성장저해 효과
Fermentation Characteristics, and Antimutagenic and Anticancer Effects of Mistletoe Added Kimchi
최선미, 길정하, 전영수, 박건영,
소속 상세정보
최선미 ( Choi Sun-Mi )
부산대학교 식품영양학과
길정하 ( Kil Jeung-Ha )
부산대학교 식품영양학과
전영수 ( Jeon Young-Soo )
부산대학교 식품영양학과
박건영 ( Park Kun-Young )
부산대학교 식품영양학과
KMID : 0603520030080020098
Abstract
Fermentation characteristics, and antimutagenic and anticancer effects of mistletoe added kimchi were studied. The changes in pH, acidity and the value of prevalence test of the mistletoe added kimchi during the fermentation were determined. Ames antimutagenicity test against aflatoxin B1 (AFB1) and N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) in Salmonella typhimurium TA100, and MTT assay using HT-29 human colon carcinoma cells and AGS human gastric adenocarcinoma cells were employed to test the chemopreventive effect of the kimchis. Mistletoe added kimchis were fermented at 15oC for 1 days and then ripened at 5oC to reach optimum pH of 4.3. Mistletoe added kimchis fermented slowly compared to the control kimchi when the changes of pH and acidity of the kimchis were compared. Sour flavor of mistletoe in the kimchis was decreased during the fermentation. Mistletoe added kimchis exhibited higher inhibitory effect against the mutagenicities induced by AFB1 and MNNG in the Ames test than the control kimchi. Especially, 2% mistletoe added kimchi showed the highest inhibitory effect (75%, 68%) in the Ames test (p＜0.05). Two % mistletoe added kimchi showed the highest anticancer effect against HT-29 human colon cells (p＜0.05). Mistletoe added kimchis exhibited significantly higher anticancer effect than the control kimchi in AGS cells, too, however, there was no significant different effect among the different levels of mistletoe (0.5～ 2.0%) addition (p＜0.05). It can be concluded that addition of mistletoe in kimchi increased preservation period of the kimchi and enhanced antimutagenic and anticancer effects of the kimchi.
키워드
Kimchi; Mistletoe; Ames mutagenicity test; MTT assay
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