Hydrogen Peroxide와 12-O-Tetradecanoylphorbol-13-actate에 의해 억제된 Gap Juctional Intercellular Communication에 대한 배추 추출물의 항암효과
The Anti-carcinogenic Effect of Korean Cabbage Extracts on the Inhibition of Gap Juctional Intercellular Communication by Hydrogen Peroxide and 12-O-Tetradecanoylphorbol-13-acetate
황재웅, 정지원, 안남식, 양세란, 박준석, 조은혜, Yoon Byong-Su, 김성훈, 이용순, 강경선,
소속 상세정보
황재웅 ( Hwang Jae-Woong )
서울대학교 수의과대학 공중보건학교실
정지원 ( Jung Ji-Won )
서울대학교 수의과대학 공중보건학교실
안남식 ( Ahn Nam-Shik )
서울대학교 수의과대학 공중보건학교실
양세란 ( Yang Se-Ran )
서울대학교 수의과대학 공중보건학교실
박준석 ( Park Joon-Suk )
서울대학교 수의과대학 공중보건학교실
조은혜 ( Jo Eun-Hye )
서울대학교 수의과대학 공중보건학교실
( Yoon Byong-Su )
경기대학교 자연과학부 생물학과
김성훈 ( Kim Sung-Hoon )
경희대학교 동서의학대학원
이용순 ( Lee Yong-Soon )
서울대학교 수의과대학 공중보건학교실
강경선 ( Kang Kyung-Sun )
서울대학교 수의과대학 공중보건학교실
KMID : 0603520030080020124
Abstract
Modulation of gap junctional intercellular communication (GJIC) is a known cellular event associated with tumor promotion. We examined the anti-carcinogenic properties of the Korean cabbage extracts (DF-01, DF-02, DF-03, Seoul, GM-A, GM-B) on the inhibition of GJIC by hydrogen peroxide (H2O2) or phorbol ester (12-O-Tetradecanoylphorbol-13-acetate; TPA) in WB-F344 rat liver cell (WB cells). The cells were pre-incubated with Korean cabbage extracts within non-cytotoxic concentration for 24 h followed by co-treatment with extracts and H2O2 (750μM) or TPA (10 ng/ml) for 1 h. DF-01, DF-02, and DF-03 prevented the inhibition of GJIC by H2O2. Inhibition by TPA of GJIC was not prevented by any extracts. To find out the action mechanism of Korean cabbage extracts, western blot analysis was performed to show whether, DF-01 and DF-03 block connexin 43 hyperphosphorylation or inhibit extracellular signal-regulated protein kinases (ERK) 1/2 activation by H2O2 in WB cells. Korean cabbage extracts such as DF-01 and DF-03 prevented H2O2 induced GJIC inhibition through inactivation of ERK1/2 mitogen-activated protein (MAP) kinases. Therefore, it is suggested that the Korean cabbage extracts might have the potential chemopreventive effects by the inhibition of GJIC.
키워드
Korean cabbage extracts; Gap junctional intercellular communication(GJIC); WB-F344 rat liver cells (WB cells); Connexin 43; Extracellular signal-regulated protein kinase (ERK)1/2
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