Conjugated Linoleic Acid(CLA)와 그 이성체가 대장암 세포인 Caco-2 세포의 Apoptosis에 미치는 영향
Effect of Conjugated Linoleic Acid and Its Isomers on the Apoptosis of Caco-2 Colon Cancer Cells
김은지, 윤정한,
소속 상세정보
김은지 ( Kim Eun-Ji )
한림대학교 생명과학부
윤정한 ( YoonPark Jung-Han )
한림대학교 생명과학부
KMID : 0603520030080030151
Abstract
Conjugated linoleic acid (CLA) is a group of positional and geometric isomers of linoleic acid (LA) and has chemoprotective properties in a variety of experimental cancer models. Cis-9,trans-11 and trans-10,cis-12 CLA are the principal isomers found in foods. The present study was performed to determine whether a mixture of CLA isomers and the two individual CIA isomers induce apoptotic cell death in Caco-2 cells. Caco-2 cells were incubated in serum-free medium containing 100 pM of the mixture of CLA isomers, 100 ,aM linoleic acid, 10 uM cis-9,trans-1 1 CLA, or 10 pM trans-10,cis-12 CLA. The mixture of CLA isomers and trans-10,cis-12 CLA inhibited Caco-2 cell growth. By contrast cis-9,trans-11 CLA had no effect. Cells treated with the mixture of CLA isomers and trans-10,cis-12 CLA produced a distinct oligonucleosomal ladder with different sizes of DNA fragments, a typical characteristic of cells undergoing apoptosis and were stained with Annexin-V. The mixture of CLA isomers and trans-10,cis-12 CLA increased the activity of caspase-3, which is a central regulator of apoptosis. These results suggested that the growth inhibitory effect of the mixture of CLA isomers may, at least in part, be attributed to caspase-3 dependent apoptosis in Caco-2 cells.
키워드
Conjugated linoleic acid(CLA);trans-10;cis-12 CLA;Apoptosis;Caspase-3
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