건선에서 감별전개 역전사중합효소 연쇄반응(DDRT-PCR)법을 이용한 펩티딜글라이신 알파-아미데이팅 모노 옥시즌네이즈(PAM)의 동정
Identification of Human Peptidylglycine Alpha-amidating Monooxygenase(PAM) in Posriasis by DDRT-PCR
권유욱,
소속 상세정보
권유욱 ( Kwon Yoo-Wook )
Laboratory of Immunopathology, National Institute of Health, Bethesda, U.S.A.
KMID : 0603520030080030195
Abstract
Psoriasis is a chronic relapsing and angiogenic skin disease characterized by variable clinical features. However, not only pathogenetic process but also etiological factors remain to be proven, In order to identify genes differentially expressed in psoriasis, we performed mRNA differential display analysis using total RNA extracted from normal and psoriatic primary cultured cells. Of the differentially expressed genes from psoriasis, some of cDNA fragments were cloned and sequenced. The expression patterns of these clones from psoriatic primary cells were confirmed by reverse northern blot analysis. Up-regulated gene from lesional keratinocytes of psoriasis was identified as human peptidylglycine alpha-amidating monooxygenase (PAM). Up-regulated expression of PAM in psoriasis may contribute to pathogenesis of psoriasis.
키워드
Psoriasis;PAM;Differential display analysis
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