Apoptosis 유발에 의한 β-lapachone 의 인체폐암세포 증식 억제에 관한 연구
Growth Inhibition of Human Lung Carcinoma Cells by β-lapachone through Induction of Apoptosis
이재훈, 박철, 최병태, 이원호, 최영현,
소속 상세정보
이재훈 ( Lee Jae-Hun )
동의대학교 한의과대학 생화학교실
박철 ( Park Cheol )
동의대학교 한의과대학 생화학교실
최병태 ( Choi Byung-Tae )
동의대학교 한의과대학 해부학교실
이원호 ( Lee Won-Ho )
부산대학교 생물학과
최영현 ( Choi Yung-Hyun )
동의대학교 한의과대학 생화학교실
KMID : 0603520040090010026
Abstract
The DNA topoismerase I inhibitor β-lapachone, the product of a lapacho tree (Tabebuia avellanedae) from South America, activates a novel apoptotic response in a number of cell lines. In the present report, we investigated the effects of β-lapachone on the growth of human lung in human non-small-cell-lung-cancer A549 cells. Upon treatment with β-lapachone, a concentration-dependent inhibition of cell viability and cell proliferation was observed as measured by hemocytometer counts and MTT assay. The β-lapachone- treated cells developed many of the hallmark features of apoptosis, including membrane shrinking, condensation of chromatin and DNA fragmentation. These apoptotic effects of β-lapachone in A549 cells were associated with a marked induction of pro-apoptotic Bax expression, however the levels of anti-apoptotic Bcl-2 expression were decreased in a dose-dependent manner. Accordingly, elevated amount of cyclindependent kinase inhibitor p21 expression accompanied by up-regulation of tumor suppressor p53 was observed. By RT-PCR analyses, decrease in gene expression level of telomerase reverse transcriptase and telomeric repeat binding factor were also observed. Thus, these findings suggest that β-lapachone may be a potential anti-cancer therapeutics for the control of human lung cancer cell model.
키워드
β-lapachone ;Lung carcinoma; Apoptosis; Bax; Bcl-2
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