왕겨 추출물의 산화적 DNA 손상 보호 효과
Effect of Rice Hull Extracts on Oxidative DNA Damage in Human Lymphocytes
전경임, 박해룡, 이승철, 박은주,
소속 상세정보
전경임 ( Jeon Kyung-Im )
경남대학교 식품영양학과
박해룡 ( Park Hae-Ryong )
경남대학교 식품생명학과
이승철 ( Lee Seung-Cheol )
경남대학교 식품생명학과
박은주 ( Park Eun-Ju )
경남대학교 식품영양학과
KMID : 0603520040090020092
Abstract
Rice hull is known to contain an antioxidant defense system to protect rice seed from oxidative stress. The aim of this study was to evaluate the effect of methanol extract of rice hull on oxidative DNA damage induced by H2O2 in human lymphocytes. Cells incubated in medium with 1 % DMSO (negative control) or with various concentration of rice hull extracts (12.5, 25, 50, 100μg/ml) for 30 min at 37oC were further treated with H2O2 (200μM) as an oxidative stimulus for 5 min on ice. Oxidative damage was evaluated by Comet assay (single-cell gel electrophoresis) and quantified as % tail DNA. Cell viability measured by trypan blue exclusion test was above 95% for all treatments. An increased oxidative DNA damage by H2O2 expressed by % tail DNA was significantly inhibited by pre-incubating with rice hull extracts in a dose dependent manner. When human lymphocytes were post-incubated with rice hull extract for 30 min after exposure to H2O2 or incubated with rice hull and H2O2 simultaneously, the protective ability of the rice hull was not changed. These results indicate that rice hull supplementation to human lymphocytes followed by H2O2 treatment inhibited damage to cellular DNA, supporting a protective effect of rice hull against oxidative damage.
키워드
Rice hull extract;DNA damage;Comet assay;Hydrogen peroxide;Lymphocytes
원문 및 링크아웃 정보
등재저널 정보