Role of Relish, a Drosophila NF-κB Protein, in Response to UV Irradiation
김영신, 양동진, 남혁진, 박소영, 김병기, 유미애,
소속 상세정보
김영신 ( Kim Young-Shin )
부산대학교 유전공학연구소
양동진 ( Yang Dong-Jin )
부산대학교 분자생물학과
남혁진 ( Nam Hyuck-Jin )
이화여자대학교 분자생명과학부
박소영 ( Park So-Young )
부산대학교 분자생물학과
김병기 ( Kim Byeong-Gee )
부산대학교 자연과학대학 생물학과
유미애 ( Yoo Mi-Ae )
부산대학교 분자생물학과
KMID : 0603520040090040237
Abstract
NF-κB has emerged as a central component of the cellular signaling machinery that serves as an important regulatory role in inflammation, immunity, and oncogenesis. During UV exposure, a positive correlation between NF-κB/Rel activation and its translocation to the nucleus, and phosphorylation and degradation of I-κB protein have been known in mammal. While the mechanisms involved in NF-κB activation upon inflammatory response are well documented both in flies and mammals, little is known that Drosophila NF-κB plays any functional roles in UV irradiation. In this study, using a null mutant of Relish, Drosophila NF-κB, we investigated a role of Drosophila NF-κB in response to UV irradiation. We found UV-induced NF-κB activation through gel mobility shift assay with the κB binding sequences of defensin promoter region. Relish null mutant larvae were more sensitive to UV-induced pigmentation, immunosuppression and apoptosis of hemocytes than wild-type. These results suggest that Drosophila NF-κB protein is involved in immune response and serves as an anti-apoptosis factor against UV irradiation.
키워드
NF-κB;Relish;UV;Drosophila
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