In Vitro Comet Assay를 이용한 수입산 녹용과 국산 녹용의 DNA 손상 보호 효과 비교 연구
The Inhibitory Effect of Antler on DNA Damage Using in Vitro Comet Assay
박유경, 전은재, 강명희,
소속 상세정보
박유경 ( Park Yoo-Kyoung )
경희대학교 동서의학대학원 의학영양학과
전은재 ( Jean Eun-Jae )
한남대학교 식품영양학과
강명희 ( Kang Myung-Hee )
한남대학교 식품영양학과
KMID : 0603520040090040253
Abstract
The growing antler has been used for the treatment of various diseases in oriental medicine. Many reports made it evident that deer antlers have various effects: anti-inflammatory effects, anti-stress activities and anti-aging activities. This study was designed to investigate the anti-oxidant activity of antler extract or antler extractherbal mixture. In addition, the anti-oxidant effect was compared between Korean antler and Russian antler. Human lymphocyte cells were treated with freeze-dried antler extract mixture at several doses that does not induce cytotoxicity (250, 1000, 20,000?/ml) for one hour at 37oC. Cells were harvested and then treated with 100? of H2O2 for 5 min at 4oC as an oxidative stress. Evaluation of the oxidative damage was performed by Comet assay (single-cell gel electrophoresis). Quantification was done by analyzing the tail moment of fragmented DNA migrated in the tail and then were expressed as relative score in relation to the damage of the positive control. Compared to the negative control cells, H2O2 treated cells showed a considerable DNA damage to the cells. Pre- treatment of antler extract mixture at a dose of 250, 1,000, 20,000?/ml reduced the oxidative damage for 43.6, 56.8, 87.7%, respectively. The protective effect on DNA damage was greater in Korean antler (62.7%) than Russian antler (31.2%) and overall protective effect was higher in the antler alone than antler mixtures. These results indicate that antler treatment to human lymphocyte cells followed by oxidative stimulus (H2O2) inhibited damage to cellular DNA, supporting a possible protective role against oxidative damage induced by reactive oxygen species.
키워드
Deer antler;DNA damage;Comet assay;Human lymphocyte cell
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