대두분말이 1,2-Dimethylhydrazine을 처리한 흰쥐의 대장 암화과정에 미치는 영향
Preventive Effects of Soypowder on Colon Carcinogenesis in 1,2-Dimethylhydrazine Treated Rats
신정은, 김인혜, 조영미, 이헌옥, 김대중, 강진석, 신원선, 엄애선,
소속 상세정보
신정은 ( Shin Jung-Eun )
Hanyang University School of Human Ecology Department of Food & Nutrition
김인혜 ( Kim In-Hye )
Hanyang University School of Human Ecology Department of Food & Nutrition
조영미 ( Cho Young-Mi )
Hanyang University School of Human Ecology Department of Food & Nutrition
이헌옥 ( Lee Heon-Ok )
Hanyang University School of Human Ecology Department of Food & Nutrition
김대중 ( Kim Dae-Joong )
Chungbuk National University College of Veterinary Medicine
강진석 ( Kang Jin-Seok )
National Institute of Toxicological Resaerch General Toxicology Department
신원선 ( Shin Weon-Sun )
Korean Food Research Institute
엄애선 ( Om Ae-Son )
Hanyang University School of Human Ecology Department of Food & Nutrition
KMID : 0603520040090040267
Abstract
This study was designed with sixty 21-day old male Fisher 344 rats to investigate the preventive effect of the soypowder on colon cancer. The colon cancer was induced by injections with colon carcinogen 1,2-dimethylhydrazine (20 mg DMH/kg body weight/time) at 4, 5, 6 and 7 weeks of age. All the rats were divided into control and experimental
groups (n=15/group, soypowder (SP)). The rats were freely fed normal and experimental diets containing 5% soypowder for 8 weeks. On 8 weeks later all the rats were sacrificed. Body weight, food consumption weight, cecum pH, aberrant crypt (AC), aberrant crypt foc (ACF) were detected. Inducible nitric oxide synthase (iNOS), cyclooxygenase-2
(COX-2), nitric oxide (NO) and histochemistry were analyzed. Body weight, food consumption, cecum pH did not differ among control and experimental groups. The number of AC and ACF in experimental groups was significantly lower than that of in control group (p＜0.05). The expression of iNOS and COX-2 in experimental groups was markedly lower than that of in control group. The concentration of NO in experimental group was significantly lower than that in control group (p＜0.05). Colon tissues were morphologically changed in control group showing damage in cell membrane and irregular arrangement of crypt. These results show that soypowder may inhibit colon carcinogenesis through diverse mechanism. Therefore, further study should be needed to identify the mechanisms by which soy powder may inhibit colon cancer.
키워드
Soypowder, Colon, Carcinogenesis, 1,2-Dimethylhydrazine
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