Pycnogenol이 HT29 결장암세포의 성장과 Inducible Nitric Oxide Synthase (iNOS) mRNA의 발현에 미치는 영향
Effect of Pycnogenol on the Growth Inhibition of HT29 Colon Adenocarinoma Cells and Inducible Nitric Oxide Synthase (iNOS) mRNA Expression
이정민,
소속 상세정보
이정민 ( Lee Jeong-Min )
남부대학교 식품영양학과
KMID : 0603520040090040274
Abstract
Pycnogenol (PYC) is a standardized extract from the French maritime pine bark that consists of a concentrate of polyphenols. The purpose of this study was to investigate the effect of PYC on the growth inhibition of HT29 colon adenocarcinoma cells and iNOS mRNA expression level. PYC treatment at 30μg/ml for 24 hr significantly induced the growth inhibition of HT29 cells. It was observed to occur in a dose-dependent manner. PYC treatment at 50 and 100μg/ml has inhibited HT29 cell growth by 83% and 98%, respectively. In estimation of iNOS mRNA expression level by RT-PCR, it was induced at 30μg/ml and increased by increment of PYC concentration. We assume that PYC treatment may, at least in part, attribute to induce iNOS to generate peroxynitrite (ONOO) resulting in the growth inhibition of HT29 cells.
키워드
Pycnogenol;HT29;iNOS;Growth inhibition;Peroxynitrite (ONOO)
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