Isoflavones이 Azoxymethane을 투여한 F344 Rats의 대장 암화과정에 미치는 영향
Preventive Effects of Isoflavones on Colon Carcinogenesis in Azoxymethane Treated F344 Rats
신정은, 심재영, 김인혜, 조영미, 문지혜, 이헌옥, 김재현, 전향숙, 엄애선,
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신정은 ( Shin Jung-Eun )
한양대학교 식품영양학과
심재영 ( Shim Jae-Young )
한양대학교 식품영양학과
김인혜 ( Kim In-Hye )
한양대학교 식품영양학과
조영미 ( Cho Young-Mi )
한양대학교 식품영양학과
문지혜 ( Moon Ji-Hea )
한양대학교 식품영양학과
이헌옥 ( Lee Heon-Ok )
한양대학교 식품영양학과
김재현 ( Kim Jae-Hyoun )
동덕여자대학교 보건관리학과
전향숙 ( Chun Hyang-Sook )
한국식품개발연구원
엄애선 ( Om Ae-Son )
한양대학교 식품영양학과
KMID : 0603520040090040280
Abstract
This study was designed with sixty 21-day old male Fisher 344 rats to investigate the preventive effect of the isoflavones on colon cancer. The colon cancer was induced by injections with colon carcinogen azoxymethane (15 mg AOM/kg body weight/time) at 4 and 5 weeks of age. All the rats were divided into control and experimental groups (n=15/group, isoflavones 30 ppm and 300 ppm). The rats were freely fed normal diets and isoflavones by oral feeding for 5 weeks. On 5 weeks later all the rats were sacrificed. Body weight, food consumption weight, cecum pH, aberrant crypt (AC), aberrant crypt foci (ACF) were detected. Inducible nitric oxide synthase (iNOS), matrix metalloproteinase (MMP)-2/9, nitric oxide (NO) were analyzed. Body weight, food consumption did not differ among control and experimental groups. The cecum pH and number of AC/ACF in experimental groups was significantly lower than that of in control group (p＜0.05). The expression of iNOS and MMP-2/9 in experimental groups was markedly lower than that of in control group. The concentration of NO in experimental groups was lower than that in control group. These results show that isoflavones may inhibit colon carcinogenesis through diverse mechanism. Therefore, further study should be needed to identify the mechanisms by which isoflavones may inhibit colon cancer.
키워드
Isoflavones;Colon;Carcinogenesis;Azoxymethane
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