스트렙토조토신 유도성 당뇨쥐의 췌장 선포세포에서 Cholecystokinin이 유도한 Calcium Signal의 변화
Altered Cholecystokinin-induced Calcium Signal in Streptozotocin-induced Diabetic Rat Pancreatic Acini
소속 상세정보
김명준/Kim MJ
류경렬/성종호/민도식/이덕주/윤신희/한상준/조양혁/김명석/Ryu GR/Sung JH/Min DS/Rhie DJ/Yoon SH/Hahn SJ/Jo YH/Kim MS
KMID : 0614620030420060519
Abstract
BACKGROUND/AIMS: Pancreatic acini of streptozotocin (STZ)-induced diabetic rats release amylase less than normal acini on cholecystokinin (CCK) stimulation. Pancreatic enzyme secretion has been closely related to the intracellular calcium concentration ([Ca2+]i) of the acinar cell. In the present study, sequential changes of the intracellular calcium signal which probably underlie the altered enzyme secretion in response to CCK-8 were investigated using pancreatic acini from diabetic rats.
METHODS: Diabetic rats were prepared by single intravenous injection of STZ (70 mg/kg). Stimulating experiments with CCK-8 were performed 7 days later. Pancreatic acini were isolated by collagenase digestion. Amylase release and [Ca2+]i were measured by colorimethod and calcium imaging, respectively. The geometry of intracellular calcium signal was analyzed.
RESULTS: Normal acini exhibited concentration-dependent [Ca2+]i increase and regular oscillatory calcium signal on CCK-8 stimulation. Amylase release was also concentration-dependent. However, diabetic acini showed significantly less [Ca2+]i increase, prolonged time to peak [Ca2+]i, decreased calcium spikes number, and decreased amylase release compared with normal acini. The decreased [Ca2+]i in diabetic acini was restored significantly by insulin treatment.
CONCLUSIONS:Relatively decreased amylase release in diabetic pancreatic acini in response to CCK, appears to be associated with altered calcium signal due to insulin deficiency.
키워드
Streptozotocin; 췌장선포; [Ca2+]i; Cholecystokinin; 아밀라제; Pancreatic acini; Amylase
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