오리고기중의 Adenosine Triphosphate Creatine Phosphotranseferase에 관한 연구(Ⅰ)
Studies on Adenosine Triphosphate Creatine Phospho-transeferase from Muscle of the Duck
광주보건전문대학논문집 1982년 7권 1호 p.31 ~ 38
소속 상세정보
남현근/Nam HK
KMID : 0615219820070010031
Abstract
오리를 시중에서 구입하여 도살하고 breast muscle을 취하여 Creatine Kinase를 추출하여 specific activity를 결정하여 76.32 units/㎎을 얻었으며, Thiol group은 6.2mol/M of enzyme을 갖고 있음을 알았다. 한편 Amino acid는 모든 필수아미노산이 함유되어 있음을 알았다.
A relatively simple procedure was devised for the purification of Adenosine Triphosphate Creatine Phosphotranseferase(E. C. 2. 7. 3. 2.) from duck breast muscle extracts. The enzyme was purified and yield of close to 25%. The specific enzyme activity, thiol group and amino acids were determined.
The results are as follows:
1. Adenosine Triphosphate Creatine Phosphotranseferase from (luck breast muscle extract has a specific activity of 76.32 units/㎎.
2. The thiol group of the duck breast muscle creatine kinase was 6.2 ㏖/M. of enzyme.
3. All most essential amino acids are contained in this enzyme.
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