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A Study on the isolation and restriction enzyme of DNA
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±è»ó¿±/Kim SY
¹Ú¹ÎÈñ/Park MH
KMID : 0615219930180010317
Abstract
The pBN18 of the gene fusion vector was double digestion with Hind¥² and EcoR¥° for the analysis of the restriction enzyme sites.
As a results, the fragments of 1.2Kb and 4.6Kb were made. Therefore it was conformed that pBN18 includes the sites of Hind¥² and EcoR¥°. The pBN18 contained the Cla¥° site to digestion with Cla¥° restriction enzyme.
A study was conformed the restriction enzyme sites of pBN18, pBN18A and pBN18B and DNA sizes of pBN18 and pBN18B were compared with the agarose gel of the electrophoresis.
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