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    Effects of microRNA-135a on the epithelial?mesenchymal transition, migration and invasion of bladder cancer cells by targeting GSK3β through the Wnt/β-catenin signaling pathway

    Experimental & Molecular Medicine 2018년 50권 1호 p.15 ~ 15
    Mao Xia-Wa, Xiao Jia-Quan, Li Zhong-Yi, Zheng Yi-Chun, Zhang Nan,
    소속 상세정보
     ( Mao Xia-Wa ) 
    Zhejiang University Medical College Second Affiliated Hospital Department of Urology Surgery
     ( Xiao Jia-Quan ) 
    Zhejiang University Medical College Second Affiliated Hospital Department of Urology Surgery
     ( Li Zhong-Yi ) 
    Zhejiang University Medical College Second Affiliated Hospital Department of Urology Surgery
     ( Zheng Yi-Chun ) 
    Zhejiang University Medical College Second Affiliated Hospital Department of Urology Surgery
     ( Zhang Nan ) 
    Zhejiang University Medical College Second Affiliated Hospital Department of Urology Surgery
    KMID : 0620920180500010015    DOI : 10.1038/emm.2017.239

    Abstract


    This study investigated the effects of microRNA-135a (miR-135a) targeting of glycogen synthase kinase 3β (GSK3β) on the epithelial?mesenchymal transition (EMT), migration and invasion of bladder cancer (BC) cells by mediating the Wnt/β-catenin signaling pathway. BC and adjacent normal tissues were collected from 165 BC patients. Western blotting and quantitative real-time PCR were used to detect the expression of GSK3β, β-catenin, cyclinD1, E-cadherin, vimentin and miR-135a in BC tissues and cells. Cells were assigned to blank, negative control (NC), miR-135a mimics, miR-135a inhibitors, small interfering RNA (siRNA)-GSK3β or miR-135a inhibitors+siRNA-GSK3β groups. miR-135a, β-catenin, cyclinD1 and vimentin expression increased, while GSK3β and E-cadherin expression decreased in BC tissues compared with adjacent normal tissues. Compared with the blank and NC groups, the expression of miR-135a, β-catenin, cyclinD1 and vimentin was higher, and cell proliferation, migration, invasion and tumor growth were increased in the miR-135a mimics and siRNA-GSK3β groups. These groups showed an opposite trend in GSK3β and E-cadherin expression and cell apoptosis. The miR-135a inhibitors group was inversely correlated with the blank and NC groups. It was concluded that miR-135a accelerates the EMT, invasion and migration of BC cells by activating the Wnt/β-catenin signaling pathway through the downregulation of GSK3β expression.

    키워드

    Bladder cancer

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