Fluoroscopic Characterization of Colonic Dysmotility Associated to Opioid and Cannabinoid Agonists in Conscious Rats
Diaz-Ruano Susana, Lopez-Perez Ana E, Giron Rocio, Perez-Garcia Irene, Martin-Fontelles Maria I, Abalo Raquel,
소속 상세정보
( Diaz-Ruano Susana )
Hospital General Universitario Gregorio Maranon Servicio de Anestesiologia
( Lopez-Perez Ana E )
Hospital General Universitario Gregorio Maranon Servicio de Anestesiologia
( Giron Rocio )
Universidad Rey Juan Carlos Facultad de Ciencias de la Salud Departamento de Ciencias Basicas de la Salud
( Perez-Garcia Irene )
Universidad Rey Juan Carlos Facultad de Ciencias de la Salud Departamento de Ciencias Basicas de la Salud
( Martin-Fontelles Maria I )
Universidad Rey Juan Carlos Facultad de Ciencias de la Salud Departamento de Ciencias Basicas de la Salud
( Abalo Raquel )
Universidad Rey Juan Carlos Facultad de Ciencias de la Salud Departamento de Ciencias Basicas de la Salud
Abstract
Background/Aims: Gastrointestinal adverse effects have a major impact on health and quality of life in analgesics users. Non-invasive methods to study gastrointestinal motility are of high interest. Fluoroscopy has been previously used to study gastrointestinal motility in small experimental animals, but they were generally anesthetized and anesthesia itself may alter motility. In this study, our aim is to determine, in conscious rats, the effect of increasing doses of 2 opioid (morphine and loperamide) and 1 cannabinoid (WIN 55,212-2) agonists on colonic motility using fluoroscopic recordings and spatio-temporal maps.
Methods: Male Wistar rats received barium sulfate intragastrically, 20-22 hours before fluoroscopy, so that stained fecal pellets could be seen at the time of recording. Animals received an intraperitoneal administration of morphine, loperamide, or WIN 55,212-2 (at 0.1, 1, 5, or 10 mg/kg) or their corresponding vehicles (saline, Cremophor, and Tocrisolve, respectively), 30 minutes before fluoroscopy. Rats were conscious and placed within movement-restrainers for the length of fluoroscopic recordings (120 seconds). Spatio-temporal maps were built, and different parameters were analyzed from the fluoroscopic recordings in a blinded fashion to evaluate colonic propulsion of endogenous fecal pellets.
Results: The analgesic drugs inhibited propulsion of endogenous fecal pellets in a dose-dependent manner.
Conclusions: Fluoroscopy allows studying colonic propulsion of endogenous fecal pellets in conscious rats. Our method may be applied to the non-invasive study of the effect of different drug treatments and pathologies.
키워드
Analgesics, opioids; Cannabinoids; Colonic motility; Fluoroscopy; Rats
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