소아 크론병의 장 점막 조직에서 FOXP3+T세포와 TGF-?1 발현에 관한 연구
FOXP3+T Cells and TGF-?1 in Colonic Mucosa of Children with Crohn’s Disease
길주현, 오정은, 서정완, 조민선, 조기영, 유은선,
소속 상세정보
길주현 ( Gil Joo-Hyun )
이화여자대학교 의학전문대학원 소아과학교실
오정은 ( Oh Jung-Eun )
이화여자대학교 의학전문대학원 소아과학교실
서정완 ( Seo Jeong-Wan )
이화여자대학교 의학전문대학원 소아과학교실
조민선 ( Cho Min-Sun )
이화여자대학교 의학전문대학원 병리학교실
조기영 ( Cho Ky-Young )
이화여자대학교 의학전문대학원 소아과학교실
유은선 ( Yoo Eun-Sun )
이화여자대학교 의학전문대학원 소아과학교실
KMID : 0816120110140030258
Abstract
Purpose: Forkhead box protein 3 (FOXP3)+T cells are the major regulatory T cells controlling all aspects of the immune response. Transforming growth factor-? (TGF-?) is a suppressive cytokine which mediates the suppressive action of FOXP3+T cells. The aim of this study was to investigate the role of FOXP3+T cells, TGF-? in colonic mucosa of children with Crohn’s disease (CD).
Methods: Colonic mucosal biopsies were obtained from 10 children with CD (12∼15 years of age) and 11 control (8∼15 years of age). Frequencies of FOXP3+T, CD4+T cells and TGF-?1 expression were examined in the lamina propria (LP) and lymphoid aggregates or follicles (LA/F) by immunohistochemistry, and later evaluated by association with disease activity.
Results: In the LP of CD group, frequencies of FOXP3+T, CD4+T cells, proportion of FOXP3/CD4+T cells and TGF-?1 expression significantly increased compared to the control. In the LA/F of CD group, frequency of FOXP3+T cells, proportion of FOXP3/CD4+T cells and TGF-?1 expression significantly increased compared to the control (p＜0.05). CD4+T cells also increased compared to the control, but this finding was not significant. In the LP and LA/F of CD group, frequency of FOXP3+T cells exhibited positive correlation with CD4+T cells (p＜0.05). In the LP and LA/F of CD group, TGF-?1 expression had positive correlation with CRP, Pediatric Crohn’s Disease Activity Index, and negative correlation with hematocrit and albumin (p＜0.05).
Conclusion: Increased frequency of FOXP3+T cells and TGF-?1 expression in colonic mucosa of CD can be interpreted as a compensatory increase towards achieving down-regulation of immune responses.
키워드
T-lymphocytes;Regulatory;Crohn’s disease;FOXP3;CD4;Transforming growth factor beta;Child;Disease activity
원문 및 링크아웃 정보
등재저널 정보