Comparison of Four Commercial ELISA Kits and In-House Immunoblotting for Diagnosis of Helicobacter pylori Infection
Jeong Hoar-Lim, Jung Yang-Sook, 전진수, 염정숙, 박지숙, 서지현, 임재영, 박찬후, 우향옥, 윤희상, 고경혁, 백승철, 이우곤, 조명제, 이광호,
소속 상세정보
( Jeong Hoar-Lim )
경상대학교 의과대학 소아과학교실
( Jung Yang-Sook )
경상대학교 의과대학 소아청소년학교실
전진수 ( Jun Jin-Su )
경상대학교 의과대학 소아청소년학교실
염정숙 ( Yeom Jung-Sook )
경상대학교 의과대학 소아청소년학교실
박지숙 ( Park Ji-Sook )
경상대학교 의과대학 소아청소년학교실
서지현 ( Seo Ji-Hyun )
경상대학교 의과대학 소아청소년학교실
임재영 ( Lim Jae-Young )
경상대학교 의과대학 소아청소년학교실
박찬후 ( Park Chan-Hoo )
경상대학교 의과대학 소아청소년학교실
우향옥 ( Woo Hyang-Ok )
경상대학교 의과대학 소아청소년학교실
윤희상 ( Youn Hee-Shang )
경상대학교 의과대학 소아청소년학교실
고경혁 ( Ko Gyung-Hyuck )
경상대학교 의과대학 병리학교실
백승철 ( Baik Seung-Chul )
경상대학교 의과대학 미생물학교실
이우곤 ( Lee Woo-Kon )
경상대학교 의과대학 미생물학교실
조명제 ( Cho Myung-Je )
경상대학교 의과대학 미생물학교실
이광호 ( Rhee Kwang-Ho )
경상대학교 의과대학 미생물학교실
KMID : 0816120120150020085
Abstract
Purpose: Commercial enzyme-linked immunosorbent assay (ELISA) kits have been considered less reliable for children than for adults. The aim of this study was to compare four ELISA kits and in-house immunoblotting based on the analysis of anti-H. pylori -IgG antibody reactivity.
Methods: A total of 399 serum samples were collected at the GNU Hospital during 1998?1999. All sera were tested using ELISA and immunoblotting. Statistically significant differences were determined by the χ2 test.
Results: The overall seropositivity rates using GAP IgG, Genedia IgG, HM-CAP, Pyloriset EIA-G, and immunoblotting were 13.0%, 25.1%, 18.3%, 15.8%, and 62.9%, respectively. Immunoblotting showed a higher seropositivity rate than did all four ELISA kits in all age groups. Genedia IgG had the highest seropositivity among the ELISA kits. The seropositivity rate for children aged 13 to 18 months was lowest, and that of children aged 15 years was highest (90.0%). The seropositivity rate for children aged 7 months to 5 years was significantly lower than that for children aged 6 to 15 years among the four ELISA kits (p＜0.0001) and immunoblotting (p=0.02).
Conclusion: Immunoblotting is the most sensitive test for detection of anti-Helicobacter pylori IgG antibodies among the serological tests in this study. These results emphasize the need for standardization when commercial ELISA tests are used in different nations or in young age groups. Immunoblotting could be a suitable noninvasive assay for serodiagnosis and seroepidemiologic study of H. pylori infection in Korean children.
키워드
ELISA; Immunoblot; Helicobacter pylori infection; Children
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