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Radiation-induced Apoptosis in Developing Fetal Rat Cerebral Cortex
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KMID : 0859320030210040315
Abstract
¸ñ Àû: ¹ßÀ° ÁßÀÎ ¹é¼ ÅÂ¾Æ ´ë³ú ÇÇÁú¿¡¼ ¹æ»ç¼±Á¶»ç¿¡ ÀÇÇÑ ¾ÆÆ÷Åä½Ã½º ¹ÝÀÀ¿¡ ´ëÇÏ¿© ¾Ë¾Æº¸°íÀÚ ÇÏ¿´´Ù.
´ë»ó ¹× ¹æ¹ý: ÀӽŠ¹é¼ÀÇ ÅÂ·É 17 ³»Áö 19ÀÏ(E17¢¦E19)¿¡ ¼±Çü°¡¼Ó±âÀÇ X-ray¸¦ ÀÌ¿ëÇÏ¿© ¾î¹Ì ÁãÀÇ º¹ºÎ¿¡ ¹æ»ç¼±À» Á¶»çÇÏ¿´´Ù. ¹æ»ç¼±·®¿¡ µû¸¥ ¾ÆÆ÷Åä½Ã½º¸¦ º¸±â À§ÇÏ¿© 1, 2, 3, 4 Gy¸¦ ÀüÈÄ Á¶»ç¸éÀ¸·Î ¹æ»ç¼±Á¶»ç¸¦ ½ÃÇàÇÏ°í 5½Ã°£ ÈÄ ¹é¼ žÆÀÇ ³ú¸¦ ȹµæÇÏ¿´´Ù. ½Ã°£ °æ°ú¿¡ µû¸¥ ¾ÆÆ÷Åä½Ã½º¸¦ º¸±â À§ÇÏ¿© E17¢¦E19¿¡ 2 GyÀÇ ¹æ»ç¼±À» Á¶»çÇÑ ÈÄ 1, 3, 6, 12, 24½Ã°£¿¡ °¢°¢ ¹é¼ žÆÀÇ ³ú¸¦ ȹµæÇÏ¿´´Ù. ´ëÁ¶±ºÀº ¹æ»ç¼±À» Á¶»çÇÏÁö ¾ÊÀº ÀӽŠÁ㸦 °°Àº Á¶°ÇÇÏ¿¡¼ »çÀ°ÇÏ¿© °¢ ±º´ç 3¸¶¸®¾¿ ÇÒ´çÇÏ¿´´Ù. ¾ÆÆ÷Åä½Ã½º ¼¼Æ÷´Â ¸é¿ªÁ¶Á÷ÈÇÐÀû ¹æ¹ý(TUNEL; In situ TdT-mediated dUTP nick end labeling)À¸·Î ¿°»öÇÏ¿© °üÂûÇÏ¿´´Ù.
°á °ú: ¹é¼ ÅÂ¾Æ ´ë³ú ÇÇÁú¿¡¼ TUNEL ¾ç¼ºÀÎ ¼¼Æ÷´Â ±¤ÇÐÇö¹Ì°æÇÏ¿¡¼ ÀüÇüÀûÀÎ ¾ÆÆ÷Åä½Ã½ºÀÇ ÇüÅÂÇÐÀû Ư¡À» ³ªÅ¸³»¾ú´Ù. ´ëÁ¶±º¿¡¼´Â ´ë³ú ÇÇÁúÀÇ Àü Ãþ¿¡¼ TUNEL ¾ç¼º¼¼Æ÷¸¦ °ÅÀÇ ¹ß°ßÇÒ ¼ö ¾ø¾ú´Ù. 1 GyÀÇ ¹æ»ç¼± Á¶»ç ÈÄ 5½Ã°£¿¡ ´ë³úÇÇÁú Àü Ãþ¿¡¼ ¹Ì¾àÇÏ°Ô °üÂûµÇ¾úÀ¸³ª 2 Gy¿¡´Â Àü Ãþ¿¡¼ 1 Gy °æ¿ìº¸´Ù ´õ Áõ°¡ÇÏ¿´°í ±×Áß ³ú½Ç´ë¿Í Áß°£´ë¿¡¼ ÇÇÁú´ëº¸´Ù ´õ ¸¹ÀÌ ³ªÅ¸³µ´Ù. Á¶»çµÈ 1¢¦4 Gy ¹üÀ§¿¡¼ ¹æ»ç¼±·®ÀÌ Áõ°¡ÇÒ¼ö·Ï TUNEL ¾ç¼º¼¼Æ÷°¡ ´õ¿í Áõ°¡ÇÏ´Â °ÍÀ» º¼ ¼ö ÀÖ¾ú´Ù. 2 GyÀÇ ¹æ»ç¼± Á¶»ç ÈÄ 3½Ã°£ºÎÅÍ TUNEL ¾ç¼º¼¼Æ÷°¡ °üÂûµÇ±â ½ÃÀÛÇÏ¿© ¹æ»ç¼± Á¶»ç ÈÄ 6½Ã°£¿¡ ÃÖ°íÁ¡À» ÀÌ·ç¾úÀ¸¸ç ÀÌ´Â 24½Ã°£±îÁö Áö¼ÓµÇ¾ú´Ù.
°á ·Ð: ¹ßÀ° ÁßÀÎ ¹é¼ ÅÂ¾Æ ´ë³úÇÇÁú¿¡¼ ¹æ»ç¼±¿¡ ÀÇÇÑ ¾ÆÆ÷Åä½Ã½ºÀÇ ÀüÇüÀûÀÎ ÇüÅÂÇÐÀû Ư¡À» °üÂûÇÒ ¼ö ÀÖ¾ú´Ù. ¾ÆÆ÷Åä½Ã½º´Â ³ú½Ç´ë¿Í Áß°£´ë¿¡¼ ÇÇÁú´ëº¸´Ù ´õ ¸¹ÀÌ ³ªÅ¸³µÀ¸¸ç ÀÌ´Â Áٱ⼼Æ÷¿Í ÃʱâÀÇ ºÐȼ¼Æ÷¿¡¼ ¹æ»ç¼±Á¶»ç¿¡ ´ëÇÑ °¨¼ö¼ºÀÌ ´õ¿í ¹Î°¨ÇÔÀ» ½Ã»çÇÏ¿´´Ù.
Purpose:This study was performed to investigate apoptosis by radiation in the developing fetal rat brain.
Materials and Methods: Fetal brains were irradiated in utero between the 17th and 19th days of fetal life (E17-19) by linear accelerator. A dose of irradiation ranging from 1 Gy to 4 Gy was used to evaluate dose dependency. To test time dependency the rats were irradiated with 2 Gy and then the fetal brain specimens were removed at variable time course; 1, 3, 6, 12 and 24 hours after the onset of irradiation. Immunohistochemical staining using in situ TdT-mediated dUTP nick end labelling (TUNEL) technique was used for apoptotic cells. The cerebral cortex, including three zones of cortical zone (CZ), intermediate zone (IZ), and ventricular zone (VZ), was examined.
Results: TUNEL positive cells revealed typical features of apoptotic cells under light microscope in the fetal rat cerebral cortex. Apoptotic cells were not found in the cerebral cortex of non-irradiated fetal rats, but did appear in the entire cerebral cortex after 1 Gy irradiation, and were more extensive at the ventricular and intermediate zones than at the cortical zone. The extent of apoptosis was increased with increasing doses of radiation. Apoptosis reached the peak at 6 hours after the onset of 2 Gy irradiation and persisted until 24 hours.
Conclusion: Typical morphologic features of apoptosis by irradiation were observed in the developing fetal rat cerebral cortex. It was more extensive at the ventricular and intermediate zones than at the cortical zone, which suggested that stem cells or early differentiating cells are more radiosensitive than differentiated cells of the cortical zone.
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Radiation; Apoptosis; Rat; Brain;¹æ»ç¼±Á¶»ç; ¾ÆÆ÷Åä½Ã½º; ¹é¼; ³ú
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