TNF-α로 유도된 HaCaT 각질형성세포의 염증반응에서해죽순의 항염증 효과
Anti-inflammatory Effect of Nypa fruticans Wurmb. on tumor necrosis factor (TNF)-α-induced Inflammatory response in HaCaT cells
배기상, 박성주,
소속 상세정보
배기상 ( Bae Gi-Sang )
원광대학교 한의과대학 본초학교실
박성주 ( Park Sung-Joo )
원광대학교 한의과대학 본초학교실
Abstract
Objectives : Nypa fruticans Wurmb. (NF) have been used as a traditional medicine to treat inflammatory diseases in East-South Asia. However, it is largely undiscovered whether NF water extract could exhibit anti-inflammatory activities against tumor necrosis factor-α (TNF-α)-induced inflammatory responses on human keratinocytes, HaCaT cells. Therefore, this study was aimed to investigate the anti-inflammatory activity of NF water extract on TNF-α induced inflammatory responses in HaCaT cells.
Methods : To investigate the anti-inflammatory activites of NF water extract in HaCaT cells, the inflammatory model of HaCaT cells was established under a suitable concentration (10 ng/ml) of human TNF-α (hTNF-α). HaCaT keratinocyte cells were pre-treated with NF water extract for 1 h, and then stimulated with hTNF-α . Then, the cells were harvested to measure the inflammatory mediators such as inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and prostaglandin E2 (PGE2), and pro-inflammatory cytokine including TNF-α and interleukin (IL)-6. In addition, we examined the inhibitory mechanisms of NF, mitogen activated protein kinases (MAPKs) and inhibitory kappa B alpha (I-B)
Results : The treatment of NF inhibited the hTNF--induced elevation of iNOS, COX-2, and PGE2 in HaCaT cells. In addition, NF treatment inhibited the hTNF-α induced elevation of TNF- and IL-6. Furthermore, NF treatment inhibited the activation of MAPKs but not degradation of I-B.
Conclusions : Taken together, our result suggest that treatment of NF could inhibit the hTNF-α induced inflammatory responses via deactivation of MAPKs in HaCaT cells. This study could suggest that NF could be a beneficial agent to prevent skin damage or inflammation.
키워드
Human keratinocytes; Inflammatory mediators; Nypa fruticans Wurmb. (NF); Tumor necrosis factor-α�筬�
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