백선피(白鮮皮) 물 추출물의 급성 췌장염 보호 효과
Protective effects of Dictamni Radicis Cortex water extract on acute pancreatitis
김동욱, 배기상, 최지원, 김동구, 김명진, 송호준, 박성주,
소속 상세정보
김동욱 ( Kim Dong-Uk )
원광대학교 한의과대학 본초학교실
배기상 ( Bae Gi-Sang )
원광대학교 한의과대학 본초학교실
최지원 ( Choi Ji-Won )
원광대학교 한의과대학 본초학교실
김동구 ( Kim Dong-Gu )
원광대학교 한의과대학 본초학교실
김명진 ( Kim Myoung-Jin )
원광대학교 한의과대학 본초학교실
송호준 ( Song Ho-Joon )
원광대학교 한의과대학 본초학교실
박성주 ( Park Sung-Joo )
원광대학교 한의과대학 본초학교실
Abstract
Objectives : Dictamni Radicis Cortex (DRC) has been used as an important traditional medicine for inflammation and fungal diseases. However, the protective effect of DRC water extract on acute pancreatitis (AP) has not been deeply reported. Therefore, we aimed to evaluate the protective effects of DRC water extract on cerulein-induced AP.
Methods : AP was induced via intraperitoneal injection of supramaximal concentrations of stable cholecystokinin analogue cerulein (50 g/kg) every hour for 6 times. DRC water extract (0.05, 0.1, or 0.2 g/kg) or saline was administrated intraperitoneally 1 h before to the first injection of cerulein. The mice were sacrificed at 6 h after the final cerulein injection. Pancreas was rapidly removed for histochemical examination and myeloperoxidase (MPO) assay. In addition, polymerase chain reaction (PCR) was performed to examine mRNA levels of pro- inflammatory cytokines such as Interleukin (IL)-1, IL-6 and Tumor necrosis factor (TNF)-.
Results : Administration of DRC water extract significantly inhibited the pancreatic weight to body weight ratio, pancreas histological damages and increase of pancreatic MPO activity during cerulein-induced AP. In addition, increased pancreatic mRNA levels of IL-1, IL-6 but not TNF- were significantly inhibited by treatment of DRC water extract against cerulein-induced AP.
Conclusions : In conclusion, we have revealed that pre-treatment of DRC water extract reduces the severity of cerulein-induced AP. Accordingly, our results could give a clinical basis that DRC could be used as a drug or agent to prevent AP.
키워드
Acute pancreatitis; Cerulein; Cytokine; Dictamni Radicis Cortex
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