황련(黃連 )추출물의 만성 역류성 식도염 유발 흰쥐에 대한 개선 효과
Improving Effects on Rats with Chronic Acid Reflux Esophagitis Treatedof Coptidis Rhizoma Extrac
김수현, 노성수, 이진아, 신미래, 이아름, 구진숙, 박해진,
소속 상세정보
김수현 ( Kim Soo-Hyun )
대구한의대학교 한의과대학 본초약리학교실
노성수 ( Roh Seong-Soo )
대구한의대학교 한의과대학 본초약리학교실
이진아 ( Lee Jin-A )
대구한의대학교 한의과대학 본초약리학교실
신미래 ( Shin Mi-Rae )
대구한의대학교 한의과대학 본초약리학교실
이아름 ( Lee Ah-Reum )
호산대학교 뷰티디자인학과
구진숙 ( Koo Jin-Suk )
안동대학교 자연과학대학 생약자원학과
박해진 ( Park Hae-Jin )
대구한의대학교 한방식품영양조리학부
Abstract
Objective : Chronic acid reflux esophagitis (CARE), one of gastroesophageal reflux disease (GERD) is increasing worldwide. Coptidis rhizoma extract (CRE) is a traditional herb that cures a variety of diseases. This study was conducted to evaluate the protective effect of CR on rats with chronic acid reflux esophagitis.
Methods : The antioxidant activities were evaluated through radical scavenging assays using 2,2-diphenyl-1- picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) radical scavenging assays. CARE was surgically induced in 5-week-old male SD rats by ligating the border between forestomach and glandular portion with a 2-0 silk tie and covering the duodenum using 18-Fr Nelaton catheter. To evaluate the esophageal protective effect of CRE, rats were divided into 3 groups: Nor (normal rats), Veh (chronic acid reflux esophagitis induced rats), CR (chronic acid reflux esophagitis induced rats treated with CRE 200 ㎎/㎏ body weight).
Results : The administration of CRE significantly prevented the mucosal injury of the esophagus tissue and histological findings improved the esophageal lesion. It has been shown that inflammation is prevented by the increase of antioxidant-related factors (Nrf-2, HO-1, SOD, catalase, and GPx-1/2) through the antioxidant pathway of esophageal tissue. The administration of CRE reduced the increase of serum peroxynitrite (ONOO-) and markedly reduced the protein expression of inflammatory mediator such as NF-Bp65, p-IB, iNOS, and IL-6.
Conclusions : Overall, these results suggest that CRE administration confirmed the protective effect of esophageal mucosa, suggesting that it is a potential treatment for chronic acid reflux esophagitis.
키워드
Chronic acid reflux esophagitis; coptidis rhizoma; anti-oxidant; anti-inflammation
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