인동(忍冬) 열수 추출물의 항산화 효과 및 HCl-Ethanol로 유도된위염 동물 모델에서의 위 점막 손상 보호 효과
The Gastroprotective and Antioxidative Effects of Lonicera japonica water extract on HCl/ethanol-induced Gastric Mucosa Damage in Rats
심미옥, 이현주, 장지훈, 정호경, 양버들, 우경완, 황태연, 김선영, 노종현, 조현우,
소속 상세정보
심미옥 ( Sim Mi-Ok )
한국한의약진흥원 한약자원본부
이현주 ( Lee Hyun-Joo )
한국한의약진흥원 한약자원본부
장지훈 ( Jang Ji-Hun )
한국한의약진흥원 한약자원본부
정호경 ( Jung Ho-Kyung )
한국한의약진흥원 한약자원본부
양버들 ( Yang Beo-Dul )
한국한의약진흥원 한약자원본부
우경완 ( Woo Kyeong-Wan )
한국한의약진흥원 한약자원본부
황태연 ( Hwang Tae-Yeon )
한국한의약진흥원 한약자원본부
김선영 ( Kim Sun-Young )
한국한의약진흥원 한약자원본부
노종현 ( Nho Jong-Hyun )
한국한의약진흥원 한약자원본부
조현우 ( Cho Hyun-Woo )
한국한의약진흥원 한약자원본부
Abstract
Objective : Gastritis is a major complication of gastrointestinal disease. Lonicera japonica is used in folk medicine to treat different diseases such as exopathogenic wind-heat, epidemic febrile diseases, sores, carbuncles and some infectious diseases. Therefore, this study examined the effects of Lonicera japonica water extract (LJE) on HCl/ethanol- induced acute gastric ulceration and anti-oxidants properties.
Methods : LC-ESI-IT-TOF MS was employed for rapid identification of major compound from LJE. The antioxidant activities were evaluated through total polyphenol and flavonoid contents and radical scavenging assays and superoxide dismutase (SOD)-like activity. SD rats were randomly divided into five different groups including the normal group, ulcer group, positive group (20 ㎏/㎎ of omeprazole, ip), and experimental groups (100 ㎏/㎎ and 500 ㎏/㎎ of LJE, ip).
Results : 4,5-Dicaffeoyl quinic acid, loganic acid, secologanic acid, sweroside, loganin, vogeloside were identified based on the detection of the molecular ion with those of literature data. The LJE was possessed free radical scavenging activities such as DPPH (IC50=189.7 ㎍/㎖), ABTS (IC50=164.5 ㎍/㎖), and SOD-like activity (IC50=405.02 ㎍/㎖). Macroscopic and histological analyses showed LJE treated group were significantly reduced to an extent that it allowed leukocytes penetration of the gastric walls compared with the ulcer group. In addition, an ulcer inhibition rate and prostaglandin E2 levels were increased in rats treated with LJE.
Conclusion : The present study has demonstrated the antioxidantive and gastroprotective effect of LJE, these findings suggested that LJE has the potential for use in treatment of gastric disorders.
키워드
Lonicera japonica; antioxidative activity; gastroprotective effect
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