창출.지모.육계 복합추출물의 고지방식이 유도 당뇨병 마우스에서의항당뇨 효능 및 C2C12 골격근세포에서의 조절기전 연구
Anti-diabetic effects of the extract from Atractylodes lancea, Anemarrhena asphodeloides and Cinnamomum Cassia mixture in high fat diet-induced diabetic mice and regulation of the function in C2C12 mouse skeletal muscle cells
박기호, 강석용, 강안나, 정효원, 박용기,
소속 상세정보
박기호 ( Park Ki-Ho )
동국대학교 한의과대학 본초학교실
강석용 ( Kang Seok-Yong )
동국대학교 한의과대학 본초학교실
강안나 ( Kang An-Na )
동국대학교 한의과대학 본초학교실
정효원 ( Jung Hyo-Won )
동국대학교 한의과대학 본초학교실
박용기 ( Park Yong-Kee )
동국대학교 한의과대학 본초학교실
Abstract
Objective : This study investigated the anti-diabetic effects of DM1, a herbal mixture with Atractylodis Rhizoma, Anemarrhenae Rhizoma, and Cinnamomi Cortex in high fat diet (HFD)-induced diabetic mice and the mechanism in C2C12 mouse skeletal muscle cells.
Methods : The C57B/6 mice were fed high fat for 12 weeks, and then administrated DM1 extract (500 ㎎/㎏, p.o.) for 4 weeks. The changes of body weight, calorie and water intakes, fasting blood glucose levels and the serum levels of glucose, insulin, triglyceride, HDL-cholesterol, AST and ALT were measured in mice. The histological changes of liver and pancreas tissues were also observed by H&E stain. C2C12 myoblasts were differentiated into myotubes and then treated with DM1 extract (0.5, 1, and 2 ㎎/㎖) for 24 hr. The expression of myosin heavy chain (MHC), PGC1α, Sirt1 and NRF1, and the AMPK phosphorylation were determined in the myotubes by western blot, respectively.
Results : The DM1 extract administration significantly decreased the calorie and water intakes, glucose, triglyceride, AST and ALT levels and increased insulin and HDL-cholesterol in HFD-induced diabetic mice. DM1 extract inhibited lipid accumulation in liver tissue and improved glucose tolerance. In C2C12 myotubes, DM1 treatment increased the expression of MHC, PGC1α, Sirt-1, NRF-1 and the AMPK phosphorylation.
Conclusion : In our results indicate that DM1 can improve diabetic symptoms by decreasing the obesity, glucose tolerance and fatty liver in HFD-induced diabetic mice, and responsible mechanism is might be related with energy enhancement.
키워드
Antidiabetic Effect; Atractylodis Rhizoma; Anemarrhenae Rhizoma; Cinnamomi Cortex; C2C12 cell; High Fat Diet; Obesity; Skeletal Muscle
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