단회 혹은 반복적인 전기경련충격이 흰쥐의 해마에서 신경세포 및 성상교세포에 미치는 영향에 관한 연구
A Study on the Effects of Single and Repeated Electroconvulsive Shocks on Neurons and Astrocyes in Hippocampus of the Rats
권오영, 황세진, 정호삼, 임병훈,
소속 상세정보
권오영 ( Kwon Oh-Young )
경상대학교 의과대학 신경과학교실
황세진 ( Hwang Se-Jin )
한양대학교 의과대학 해부.세포생물학교실
정호삼 ( Chung Ho-Sam )
한양대학교 의과대학 해부학교실
임병훈 ( Lim Byeong-Hoon )
경상대학교 의과대학 신경과학교실
KMID : 0877219970010010060
Abstract
Objectived : Electroconvulsive therapy (ECT) is an effective treatment for some disorders including depression. While definitive structral damage of brain induced by ECT is still controversial evidences of neuronal damage were reported by many authors 14 has been also suggested that neuronal activation by various manners including electroconvulsive shock (ECS), could alter the astroglial gene expression. To investigate the effects of ECS on the neurons and astrocytes of hippocampus, we obserbed the changes in morphology and the immunoreactivity of glial fibrillary acidic protein(GFAP) and S-100 protein, the specific markers for astrocytes.
Subjects and Methods : Experimental animals were treated with single or repeated (once/day for 10 days) ECS (100 v. AC, for 0.9sec) and sacrificed at 1 day or 10 days after last ECS. Luxol fast blue-cresyl violet stain, GFAP and S-100 immunohistochemistry were performed.
Result : 1) No histological and immunohistochemical changes were observed in rats treated with singel ECS. 2) Neuronal atrophy and nuclear pyknosis of hippocampal pyramidal cells and dentate granular cells were observed at 1st day after repealed ECS, but these findings were not found at 10th day after repeated ECS. 3) Increase of GFAP immunoreactivity in dentate gyrus, and S-100 protein immunoreactivity in dentate gyrus and CAl area of hippocampus were observed at 1st day after repeated ECS, but these findings were not found at 10th day after repealed ECS.
Conclusion : It is consequently suggested that repealed ECS may transiently induce the morphologic change of neurons and increase of GFAP and S-100 protein immunoreactivity, the latter may be related with neuroprotection in hippocampus of the rats
키워드
전기경련요법; 해마; 신경세포의 형태학적 변화; GFAF및 S-100단백의 면역반응도; 신경세포보호
Electroconvulsive therapy; Hippocampus; Morphologic change of neurons; GFAP and S-100 protein immunoreactivity; Neuroprotection
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