Kainic Acid에 의한 흰쥐 해마의 손상과정에서 RPIA가 미치는 영향에 관한 형태학적 연구
A Morphological Study on the Effects of RPIA on Kainic Acid-induced Neurotoxicity in the Hippocampus in Rats
신동진, 황세진, 정호삼,
소속 상세정보
신동진 ( Shin Dong-Jin )
인천 중앙길병원 신경과
황세진 ( Hwang Se-Jin )
한양대학교 의과대학 해부.세포생물학교실
정호삼 ( Chung Ho-Sam )
한양대학교 의과대학 해부학교실
KMID : 0877219970010010071
Abstract
Systemic injection of kainic acid (KA) in experimental animals induces the limbic seizure and structural damages in hippocampus and amygdala which resemble the changes in human temporal lobe epilepsy. In recent reports, some adenosine analogues exert neuroprotective actions against excitatory or ischemic neurotoxicity by several mechanisms. The author performed this study to investigate the neuroprotective effects of an adenosine agonist, R-phenylisopropyl adenosine (RPIA), on the neurotoxicity induced by KA in the hippocampus in rats. Kainic acid (10 ㎎/㎏) was administered intraperitoneally, and coadministration of KA with RPIA (100 ㎍/㎏, i p. 10 min prior to injection of KA), wase also performed. The experimental animals were sacrificed at 1 day and 1 week after drug administration. Luxol frost blue cresyl violet stain and GFAP immunohistochemistry in hippocampus were performed. Loss of pyramidal cells in CA1 and CA3 of hippocampus and increase of GFAP immunoreactivity in the dentate gyrus and all hippocampal area were observed 1 day and 1 week after KA injection. Neuronal atrophy and nuclear pyknosis were observed but substantial cell loss was not found after coadministration of KA with RPIA. GFAP immunoreactivity in the hippocampus was not increased after coadministration of KA and RPIA. It is consequently suggested that RPIA may attenuate the KA-induced neuronal loss and increase of GFAP immunoreactivity in hippocampus in rats.
키워드
해마
Kainic acid; R-phenylisoprpyladenosine; Hippocampus; GFAP; Epilepsy; Neuroprotection
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