Kainic Acid 유발 흰쥐발작모델에서 (R-)-N6-phenylisopropyladenosine의 전처치가 번연계의 신경세포접착분자(Netural Cell Adhesion Molecule ; NCAM)의 면역반응변화에 미치는 영향
Effect of (R-)-N6-phenylisopropyladenosine (RPIA) Pretreatment on the alteration of Netural Cell dhesion Molecule
황선출, 황세진, 조원길, 진창배, 정호삼,
소속 상세정보
황선출 ( Hwang Seon-Chool )
메리놀병원 신경과
황세진 ( Hwang Se-Jin )
한양대학교 의과대학 해부.세포생물학교실
조원길 ( Cho Won-Gil )
한양대학교 의과대학 해부학교실
진창배 ( Jin Chang-Bae )
한국과학기술연구원 생체대사연구센터
정호삼 ( Chung Ho-Sam )
한양대학교 의과대학 해부학교실
KMID : 0877219990030010022
Abstract
Background:Various neuronal and glial factors which participate in neural differentiation, including neural cell adhesion molecule (NCAM), are upregulated in pathogenesis of temporal lobe epilesy (TLE).This study aimed to investigate hte effect of (R-)-N6-phenylisopropyladenosine (RPIA), an adenosine A1 receptor agonist, on the morphological alteration of NCAM immunoreactivity (IR) in limbic system of Kainic acid (KA)-induced epileptic rats.
Methods:Experiment animals were divided into control group, KA treatment only (10 ㎎/㎏. i.p.)group, and RPIA pretreatment (100 ㎍/㎏. i,p, 10 min prior to injection of KA) group. Animals were sacrificed at 24 hours and 1 week after KA treatment. Luxol fast blue-cresyl violet stain for histopathological observation, and NCAM immunohistochemistry to study alteration of NCAM IR in limbic system were performed.
Results:Neuronal loss in CA1 and CA3areas of hippocampus, piridorm cortex, basolateral amygdala nucleus and lateral dorsal thalamic nucleus were induced by KA unjection, and thoes were reduced by RPIA pretreatment. Inrease of NCAM-IR was observed in interneurons of all hippocampal areas. except CA2 area, pirform cortex and basolateral amygdala nucleus at 24 hours after KA injection. and increased NCAM-IR was observed in cell membrane and processes of neuroglia, dentate granule cells and pyramidal cells in CA1 area of hippocampus. and neurons in piriform cortex, amygdala and lateral dorsal thalamic nucleus 1 week after KA injection, but those changes were milder than those at 24 hours after KA injection. RPIA pretreatment significantly reduced KA-induced NCAM-IR in hippocampal CA3, CA1 area, piriform cortex, amtgdala and lateral dorsal thalamic nucleus.
Conclusion:We suggest that decrease of NCAM immunoreactivity is associated with neuprotective effects of RPIA on limbic system against KA neurotoxiciy.
키워드
Kainic acid; (R-)-N6-phenylisopropyladenosine ; Limbic system; Netural cell adhesion molecule; Temporal lobe epilepsy
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