단회 및 반복적인 Kainic Acid의 복강내 주사 후 백서 해마 Fos단백 발현의 비교
Comparison of Fos Protein Expressions Rat Hippocampus Following Single and Repeated Intraperitoneal Administration of Kainic Acid
김광수, 유경무, 장희경,
소속 상세정보
김광수 ( Kim Kwang-Soo )
고신대학교 의과대학 신경과학교실
유경무 ( Yoo Kyung-Moo )
고신대학교 의과대학 신경과학교실
장희경 ( Chang Hee-Kyung )
고신대학교 의과대학 병리학교실
KMID : 0877219990030010033
Abstract
Background:Acute seizures that increase neuronal activity cause a rapid and transient induction of the immmediate early c-fos in specific brain regions. C-fos gene may mediate long-term changes in cell function, such as growth, differeniation, and development, in response to acute extracellular stimulation. This study is designed to compare the expression of Fos protein in hippocampus after single and repeated injections of kainic acid (KA).
Methods:In KA-single injection model, twelve adult male Sprague-Dawley rats were treated with single intraperitoneal injection of convulsive dose(20-30 mg/kg) of KA, and. in KA-multiple injections model, seven rats received KA by repeated daily intraperitoneal injections for 15 days. Eight control rats received normal saline. Expression of Fos protein was tested in hippocampus by immunohistochemical staining, and was scored by the degree of staining intensity and the ratio of stained cells to tested ones.
Results:The scores tended to increase in CA3 and dentate gyrus were significantly higher in KA-single injection model than in control (p<0.05). In comparison with scores in KA-model.
Conclusion:These results show that repeated seizure produces some blockade of c-fos induction in CA2, CA3 and dentate gyrus. This may be a long-term adaptive response by the nervous system to repated neuronal activation.
키워드
Fos protein; Rat hippocampus; Kainic acid-induced seizure
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