흰쥐 편측 해마에 카이닉산 유도 경련이 반대측 해마 신경세포 손상에 미치는 영향
Neuronal Cell Death in the Contralateral Hippocampus after Unilateral Hippocampal Kainic Acid-induced Seizure in Rats
박성경, 양동원, 이상봉, 박성민, 최재영, 김영인,
소속 상세정보
박성경 ( Park Soung-Kyeong )
가톨릭대학교 의과대학 신경과학교실
양동원 ( Yang Dong-Won )
가톨릭대학교 의과대학 신경과학교실
이상봉 ( Lee Sang-Bong )
가톨릭대학교 의과대학 신경과학교실
박성민 ( Park Seong-Min )
가톨릭대학교 의과대학 신경과학교실
최재영 ( Choi Jae-Young )
가톨릭대학교 의과대학 신경과학교실
김영인 ( Kim Yeong-In )
가톨릭대학교 의과대학 신경과학교실
KMID : 0877220010050010003
Abstract
Background: The recurrent temporal lobe epilepsy induces contralateral cell damage and secondary epileptogenesis in the contralateral hippocampus of rats. This phenomenon is fairly constant and has been used as a model of human temporal lobe epilepsy. It is necessary to understand this patho-mechanism in order to prevent this cell damage.
Methods: We have investigated the patho-mechanism of secondary epileptogenesis by using the rat model injected with kainic acid (KA) into the unilateral hippocampus. KA model shows initial complex partial seizures originating from the limbic structures and following convulsive status epilepticus. Immunohistochemical staining for c-fos expression, TUNEL stain for apoptosis, and hematoxylin-eosin (H-E) stain for morphologic changes were used.
Results: In the injected hippocampus, transient activation of c-fos was expressed in the dentate gyrus and CA3 hippocampal area, which were shaded out within 24 hours after the onset of limbic seizure. The stained cell with normal appearance was not observed in the H-E stain after 72 hours due to diffuse cell death. In the contralateral hippocampus, transient expression of c-fos was observed in the dentate gyrus, hilus, CA3, and CA1 area. But the expression of c-fos in the CA3 and CA1 area was sustained to 24 hours. Cell loss was mild in the CA3 and hilus, and mild cell degeneration and shrinkage were observed in the CA1 area. Apoptotic body was expressed in the CA1 area at 72 hours after the onset of seizure.
Conclusion: These results mean that the area of prolonged expression of c-fos is vulnerable to apoptosis. Also it suggests that the patho-mechanism of ipsilateral hippocampus is an acute cytotoxic edema, whereas the contralateral damage is an apoptosis.
키워드
카이닉 산; Fos 단백; 변연계 경련; 세포자멸사
Kainic acid; C-fos; Apoptosis; Limbic seizure
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