Flurothyl-유발 발작 감수성에 대한 케톤생성식이요법의 효과
Effect of the Ketogenic Diet on Flurothyl-Induced Seizure Susceptibility
김동욱, 채수안, 정기영, 김재문,
소속 상세정보
김동욱 ( Kim Dong-Wook )
인제대학교 의과대학 일산백병원 소아과학교실
채수안 ( Chae Soo-Ahn )
중앙대학교 의과대학 소아과학교실
정기영 ( Jung Ki-Young )
충남대학교 의과대학 신경과학교실
김재문 ( Kim Jae-Moon )
충남대학교 의과대학 신경과학교실
KMID : 0877220010050020119
Abstract
Purpose: Despite decades of clinical experience with the ketogenic diet (KD), its efficacy and mechanisms of action have been examined in few animal studies. The present study was designed to investigate the effect of a KD on flurothyl-induced seizure susceptibility in rats.
Methods: Twenty male Sprague-Dawley rats were divided into two equal groups. Dietary treatment was initiated at P39. The experimental group was fasted for a day and then fed a KD consisting of [fat] : [protein + carbohydrate] ratio of 4.3 : 1, while the control group was fed a standard rodent chow. On treatment day 21, blood beta-hydroxybutyrate (BHB) levels were assayed and seizures were chemically induced by flurothyl (40 micro l/min). Seizure susceptibility was defined as the latency from the start of flurothyl infusion to the onset of a generalized seizure (loss of posture with bilateral hindlimb tonic extension). Shorter latencies reflect greater seizure susceptibility.
Results: Blood BHB levels in the KD-treated group were significantly higher than those of the control group (4.75+/-0.38 [n=10] vs. 0.19+/-0.02 [n=10] mM, respectively ; p<0.01). The latencies to the onset of a generalized seizure were 673.2+/-32.95 [n=10] and 523.0+/-31.11 [n=10] seconds for the KD-treated and control groups, respectively (p<0.01).
Conclusion: This study demonstrates the significant decrease in the susceptibility of flurothyl-induced seizure in the KD-treated rats. Furthermore, we have established a working animal model from which future mechanistic studies can be based.
키워드
케톤생성식이요법; 발작 감수성
beta-Hydroxybutyrate; 쥐; Ketogenic diet; Flurothyl; Seizure susceptibility; beta-Hydroxybutyrate; Rat
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